CIRI - Centre International de Recherche en Infectiologie, Team GIMAP (Saint-Etienne), Université Claude Bernard Lyon 1, Inserm, U1111, CNRS, UMR5308, ENS Lyon, UJM, 69007, Lyon, France.
Boehringer Ingelheim, Global Innovation, Saint-Priest, France.
Sci Rep. 2024 Aug 31;14(1):20290. doi: 10.1038/s41598-024-71310-9.
In the human and veterinary fields, oral vaccines generate considerable interest. In dogs, these vaccines are newly developed, and understanding their mechanisms is crucial. Mesenteric lymph nodes (MLNs) and Peyer's patches (PPs) are important sites for gastrointestinal mucosal induction, yet canine MLNs lack sufficient information. To address this, we collected MLN samples from healthy dogs, performed flow cytometry to characterize immune cells, and conducted single-cell RNA sequencing (scRNA-seq) to explore subpopulations, particularly B and T lymphocytes. This effort enabled the characterization of canine MLN's main cell populations and the construction of a predictive atlas, as well as the identification of particularities of this area.
在人类和兽医领域，口服疫苗引起了相当大的兴趣。在犬类中，这些疫苗是新开发的，了解它们的机制至关重要。肠系膜淋巴结 (MLNs) 和派尔氏斑 (PPs) 是胃肠道黏膜诱导的重要部位，但犬 MLNs 缺乏足够的信息。为此，我们从健康犬中收集了 MLN 样本，进行流式细胞术以鉴定免疫细胞，并进行单细胞 RNA 测序 (scRNA-seq) 以探索亚群，特别是 B 和 T 淋巴细胞。这项工作使我们能够对犬 MLN 的主要细胞群进行特征描述，并构建一个预测图谱，同时还确定了该区域的特殊性。
