Qilu University of Technology (Shandong Academy of Science), Shandong Analysis and Test Center, Key Laboratory for Applied Technology of Sophisticated Analytical Instrument of Shandong Province, Jinan, 250014, PR China.
Qilu University of Technology (Shandong Academy of Science), Shandong Analysis and Test Center, Key Laboratory for Applied Technology of Sophisticated Analytical Instrument of Shandong Province, Jinan, 250014, PR China.
Anal Chim Acta. 2024 Oct 2;1324:343071. doi: 10.1016/j.aca.2024.343071. Epub 2024 Aug 6.
Environmental endocrine disruptors (EEDs) are a class of new pollutants that are diffusely used in the medical industry and animal husbandry. In view of toxicity concerns, elevated levels of EEDs in the environment and food, which cause potential harm to human beings and ecosystems, must be monitored. Determination of EEDs contaminants to ensure environment and food safety has became a major concern worldwide, it is also a challenging task because of their trace level and probable matrices interference. Thus, developing rapid adsorption and efficient analysis methods for EEDs is apparently necessary.
A magnetic conjugated micro-porous polymer (FeO@TbDt) was designed and synthesized, which was endowed with large specific surface area, rich functional groups and magnetic responsiveness. The material showed high extraction efficiency for EEDs via magnetic solid-phase extraction (MSPE). The quantum chemistry calculations showed the adsorption mechanism of FeO@TbDt on EEDs mainly included electrostatic interactions, van der waals forces (N-H … π interaction, C-H … π interaction), and multiple hydrogen bonds. Finally, a trace analysis method for nine EEDs was established combined with HPLC-MS/MS under optimized MSPE conditions. The method showed a good linearity (R ≥ 0.996), low limits of detection (0.25-5.1 ng L), high precision (RSD of 1.1-8.2 %, n = 6). The applicability of this method was investigated by analyzing four water samples and two dairy products, and satisfactory recovery rates (82.1-100.7 %) were obtained. The proposed method showed the potential for the analysis of EEDs residues in food and environmental samples.
The developed MSPE method based on conjugated micro-porous polymers (CMPs) is simple, green, and efficient compared to existing techniques. The application of CMPs provides a new idea for preparing versatile sample pre-treatment materials. What's more, this work has certain reference value for addressing of EEDs residues in the environment and food.
环境内分泌干扰物（EEDs）是一类在医学和畜牧业中广泛使用的新型污染物。鉴于其毒性问题，必须监测环境和食物中 EEDs 水平的升高，因为这些升高可能对人类和生态系统造成潜在危害。为了确保环境和食品安全而对 EEDs 污染物进行测定已成为全球关注的焦点，这也是一项具有挑战性的任务，因为它们的浓度很低，且可能受到基质干扰。因此，开发用于 EEDs 的快速吸附和高效分析方法显然是必要的。
设计并合成了一种磁性共轭微孔聚合物（FeO@TbDt），该材料具有大的比表面积、丰富的官能团和磁响应性。通过磁固相萃取（MSPE），该材料对 EEDs 表现出了高的萃取效率。量子化学计算表明，FeO@TbDt 对 EEDs 的吸附机制主要包括静电相互作用、范德华力（N-H…π 相互作用、C-H…π 相互作用）和多重氢键。最后，在优化的 MSPE 条件下，结合高效液相色谱-串联质谱（HPLC-MS/MS）建立了一种用于痕量分析 9 种 EEDs 的方法。该方法具有良好的线性（R≥0.996）、低检测限（0.25-5.1ngL）、高精密度（RSD 为 1.1-8.2%，n=6）。通过分析四个水样和两个乳制品样品验证了该方法的适用性，均获得了令人满意的回收率（82.1-100.7%）。该方法为分析食品和环境样品中的 EEDs 残留提供了潜力。
与现有技术相比，基于共轭微孔聚合物（CMPs）的 MSPE 方法简单、绿色、高效。CMPs 的应用为制备多功能样品预处理材料提供了新的思路。此外，这项工作对解决环境和食品中的 EEDs 残留问题具有一定的参考价值。
