Lee Jin Gyeom, Li Yuchi, Kim Nam Jun, Jang Han Byeol, Yang Chae Ha, Kim Hee Young, Yoon Seong Shoon, Chang Suchan, Jeong Seon-Ju, Kim Sang Chan, Sa Bok Suk, Lee Bong Hyo
Department of Acupuncture, Moxibustion and Acupoint, College of Korean Medicine, Daegu Haany University, Daegu, Republic of Korea.
Research Center for Herbal Convergence on Liver Disease, Daegu Haany University, Gyeongsan, Republic of Korea.
Integr Med Res. 2024 Sep;13(3):101052. doi: 10.1016/j.imr.2024.101052. Epub 2024 Jun 1.
Herbal medicine Ja-Geum-Jeong (JGJ) has been used for the treatment of detoxification in Eastern Asia. However, the mechanisms involved are not clearly defined. The purpose of the present study was to investigate if herb medication inhibits Methamphetamine (METH)'s reinforcing effect and also examined if a combination of herb medication and acupuncture produces a synergistic effect on METH.
Male Sprague-Dawley rats were given acute METH intraperitoneally and the locomotor activity and ultrasonic vocalization (USV) calls were measured. Rats were administered JGJ orally and acupuncture was given at HT7 or SI5. Monosodium glutamate (MSG) and gamma-aminobutyric acid (GABA) agonists were injected into the Central amygdala (CeA) to investigate a possible neuroscientific mechanism. Tyrosine hydroxylase (TH) and fast scan cyclic voltammetry (FSCV) were measured to immunohistochemically and electrically confirm the behavioral data.
Locomotor activity and USV calls were increased by METH ( < 0.05) and these increases were inhibited by JGJ ( < 0.05). Also, JGJ had no effect on the normal group given saline, and acupuncture at SI5 acupoint, but not at HT7 acupoint, produced a synergistic effect when combined with JGJ ( < 0.05). The JGJ's inhibition was blocked by the inactivation of CeA ( < 0.05), and MSG mimicked JGJ ( < 0.05). TH and FSCV measures showed the same pattern with the behavioral data ( < 0.05).
Results of the present study suggest that JGJ had inhibitory effects on the METH which was mediated through the activation of CeA and that combination of acupuncture and herb produced synergistic effect.
草药加金黄正（JGJ）在东亚地区一直被用于解毒治疗。然而，其作用机制尚不清楚。本研究的目的是调查草药用药是否抑制甲基苯丙胺（METH）的强化作用，并研究草药用药与针灸联合使用是否对METH产生协同作用。
对雄性Sprague-Dawley大鼠腹腔注射急性METH，测量其运动活性和超声发声（USV）叫声。大鼠口服JGJ，并在HT7或SI5进行针灸。将谷氨酸钠（MSG）和γ-氨基丁酸（GABA）激动剂注入中央杏仁核（CeA），以研究可能的神经科学机制。通过免疫组织化学和电生理方法测量酪氨酸羟化酶（TH）和快速扫描循环伏安法（FSCV），以证实行为学数据。
METH使运动活性和USV叫声增加（P<0.05），而JGJ可抑制这些增加（P<0.05）。此外，JGJ对给予生理盐水的正常组无影响，SI5穴位针刺（而非HT7穴位）与JGJ联合使用时产生协同作用（P<0.05）。CeA失活可阻断JGJ的抑制作用（P<0.05），MSG模拟了JGJ的作用（P<0.05）。TH和FSCV测量结果与行为学数据显示相同模式（P<0.05）。
本研究结果表明，JGJ对METH具有抑制作用，其作用通过激活CeA介导，且针灸与草药联合使用产生协同作用。
