Zhang Yao, Wan Youming, Qu Suping, Mu Zhengchun, Li Yuying, Li Zhenghong
Institute of Highland Forest Science, Chinese Academy of Forestry, Kunming, China.
College of Forestry, Nanjing Forestry University, Nanjing, China.
Front Plant Sci. 2024 Aug 14;15:1428364. doi: 10.3389/fpls.2024.1428364. eCollection 2024.
is a vulnerable species endemic to Yunnan Province, Southwestern China, which has high ornamental value. Its wild population has not been fully protected and utilized for a long time, which is not conducive to the long-term stable development of this species. Genetic diversity assessment is the basis and prerequisite for the conservation of rare species. In this study, 21 phenotypic traits and 17 highly polymorphic EST-SSR markers were used to analyze the genetic diversity and genetic structure of 164 individuals from six populations. The coefficient of variation of 21 phenotypic traits ranged from 11.76% to 52.58% (mean=21.72%), and the coefficient of variation of 18 traits was less than 30%. The average values of , , and were 1.710, 0.619, 0.384, and 0.352, respectively. The genetic diversity of LLO ( = 0.476 and = 0.426) and LCM ( = 0.424 and = 0.381) populations in Lushui County was highest. The GDX populations ( = 0.335 and = 0.269) isolated by Gaoligong Mountain had the lowest genetic diversity. The AMOVA results showed that 13.04% of the genetic variation was among populations and 86.96% was within populations. The average phenotypic differentiation coefficient of phenotypic traits among populations was 18.69%. The results of phenotypic and genetic variation analysis were consistent, indicating that the most of variation exists within population. Genetic structure, UPGMA clustering and PCA analysis results showed that the populations of had obvious geographical divisions, and the populations distributed in the southern region and distributed in the northern region of the Nujiang River clustered into one group respectively. Combining the results of phenotypic and molecular markers, we recommend that give priority to the protection of LLO, LCM and GDX population, in order to ensure the sustainable utilization of germplasm resources.
是中国西南部云南省特有的易危物种，具有很高的观赏价值。其野生种群长期未得到充分保护和利用，不利于该物种的长期稳定发展。遗传多样性评估是珍稀物种保护的基础和前提。本研究利用21个表型性状和17个高度多态性的EST-SSR标记，对来自6个种群的164个个体的遗传多样性和遗传结构进行了分析。21个表型性状的变异系数在11.76%至52.58%之间（平均值=21.72%），18个性状的变异系数小于30%。、、和的平均值分别为1.710、0.619、0.384和0.352。泸水县的LLO种群（=0.476和=0.426）和LCM种群（=0.424和=0.381）遗传多样性最高。被高黎贡山隔离的GDX种群（=0.335和=0.269）遗传多样性最低。AMOVA结果表明，13.04%的遗传变异存在于种群间，86.96%存在于种群内。种群间表型性状的平均表型分化系数为18.69%。表型和遗传变异分析结果一致，表明大部分变异存在于种群内。遗传结构、UPGMA聚类和PCA分析结果表明，的种群有明显的地理划分，分布在怒江以南地区和以北地区的种群分别聚为一组。结合表型和分子标记结果，建议优先保护LLO、LCM和GDX种群，以确保种质资源的可持续利用。
