Pang Yunlong, Wang Liming, Li Linzhi, Wang Xiaoqian, Wang Danfeng, Zhao Meng, Ma Chenhao, Zhang Huirui, Yan Qiang, Lu Yue, Liang Yunlong, Kong Xiangsheng, Zhu Huaqiang, Sun Xuecheng, Zhao Yujie, Liu Shubing
National Key Laboratory of Wheat Improvement, College of Agronomy, Shandong Agricultural University, Tai'an, China.
College of Agriculture, Henan University of Science and Technology, Luoyang, China.
Mol Breed. 2024 Aug 31;44(9):56. doi: 10.1007/s11032-024-01496-3. eCollection 2024 Sep.
Wheat is one of the most important staple foods in the world. Genetic characterization of wheat agronomically important traits is crucial for yield improvement through molecular breeding. In this study, a recombinant inbred line (RIL) population was developed by crossing a local adapted high yield variety Jimai 22 (JM22) with an external variety Cunmai no.1 (CM1). A high-density genetic map containing 7,359 single nucleotide polymorphism (SNP) markers was constructed. Quantitative trait loci (QTL) mapping identified 61 QTL for eight yield-related traits under six environments (years). Among them, 17 QTL affecting spike number per plant, grain number per spike and thousand grain weight showed high predictability for theoretical yield per plant (TYP), of which, 12 QTL alleles positively contributed to TYP. Nine promising candidate genes for seven of the 12 QTL were identified including three known wheat genes and six rice orthologs. Four elite lines with TYP increased by 5.6%-15.2% were identified through genotype selection which carried 7-9 favorable alleles from JM22 and 2-3 favorable alleles from CM1 of the 12 QTL. Moreover, the linked SNPs of the 12 QTL were converted to high-throughput kompetitive allele-specific PCR (KASP) markers and validated in the population. The mapped QTL, identified promising candidate genes, developed elite lines and KASP markers are highly valuable in future genotype selection to improve wheat yield.
The online version contains supplementary material available at 10.1007/s11032-024-01496-3.
小麦是世界上最重要的主食之一。对小麦农艺重要性状进行遗传特征分析对于通过分子育种提高产量至关重要。在本研究中，通过将一个当地适应性高产品种济麦22（JM22）与一个外来品种存麦1号（CM1）杂交，构建了一个重组自交系（RIL）群体。构建了一个包含7359个单核苷酸多态性（SNP）标记的高密度遗传图谱。数量性状位点（QTL）定位在六个环境（年份）下鉴定出了与八个产量相关性状的61个QTL。其中，17个影响单株穗数、穗粒数和千粒重的QTL对单株理论产量（TYP）具有较高的预测性，其中12个QTL等位基因对TYP有正向贡献。鉴定出了12个QTL中7个的9个有前景的候选基因，包括3个已知的小麦基因和6个水稻直系同源基因。通过基因型选择鉴定出了4个TYP提高5.6%-15.2%的优良品系，这些品系携带了12个QTL中来自JM22的7-9个有利等位基因和来自CM1的2-3个有利等位基因。此外，将12个QTL的连锁SNP转化为高通量竞争性等位基因特异性PCR（KASP）标记并在群体中进行了验证。所定位的QTL、鉴定出的有前景的候选基因、培育的优良品系和KASP标记在未来提高小麦产量的基因型选择中具有很高的价值。
在线版本包含可在10.1007/s11032-024-01496-3获取的补充材料。
