IL13RA2 通过激活糖酵解和 Wnt/β-catenin 信号通路促进婴儿血管瘤的进展。

IL13RA2 promotes progression of infantile haemangioma by activating glycolysis and the Wnt/β-catenin signaling pathway.

机构信息

Department of Cardiothoracic Surgery, 970th Hospital of the People's Liberation Army, Weihai, 264209, China.

Department of Cardiac Ultrasound, Weihai Municipal Hospital, Weihai, 264200, China.

出版信息

Oncol Res. 2024 Aug 23;32(9):1453-1465. doi: 10.32604/or.2024.048315. eCollection 2024.

DOI:10.32604/or.2024.048315

PMID:39220137

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC11361910/

Abstract

BACKGROUND

Interleukin 13 receptor subunit alpha 2 (IL13RA2) plays an essential role in the progression of many cancers. However, the role of IL13RA2 in infantile haemangioma (IH) is still unknown.

MATERIALS AND METHODS

IL13RA2 expression in IH tissues was analyzed using western blot, qRT-PCR, and immunofluorescence. The role of IL13RA2 in haemangioma-derived endothelial cells (HemECs) was determined following knockdown or overexpression of IL13RA2 using CCK-8, colony formation, apoptosis, wound healing, tubule formation, Transwell, and western blot.

RESULTS

IL13RA2 expression was upregulated in IH tissues. IL13RA2 overexpression promoted proliferation, migration, and invasion of HemECs and induced glycolysis, which was confirmed with a glycolysis inhibitor. Specifically, IL13RA2 interacted with β-catenin and activated the Wnt/β-catenin pathway in HemECs, which were involved in the above-mentioned effects of IL13RA2.

CONCLUSIONS

These findings revealed that targeting IL13RA2 is a potential therapeutic approach for IH.

摘要

背景

白细胞介素 13 受体亚单位 α2（IL13RA2）在许多癌症的进展中发挥着重要作用。然而，IL13RA2 在婴幼儿血管瘤（IH）中的作用尚不清楚。

材料和方法

采用 Western blot、qRT-PCR 和免疫荧光法分析 IH 组织中 IL13RA2 的表达。通过 IL13RA2 敲低或过表达，使用 CCK-8、集落形成、凋亡、划痕愈合、小管形成、Transwell 和 Western blot 测定 IL13RA2 在血管内皮细胞中的作用。

结果

IL13RA2 在 IH 组织中表达上调。IL13RA2 的过表达促进了 HemECs 的增殖、迁移和侵袭，并诱导了糖酵解，这一过程可以用糖酵解抑制剂来证实。具体来说，IL13RA2 在 HemECs 中与β-catenin 相互作用并激活 Wnt/β-catenin 通路，这涉及到 IL13RA2 的上述作用。