分析宫颈 HPV 感染、宫颈病变与阴道微生态的相关性。
Analysis of the correlation between cervical HPV infection, cervical lesions and vaginal microecology.
机构信息
Department of Urology, The Affiliated Suzhou Hospital of Nanjing Medical University, Suzhou Municipal Hospital, Gusu School, Nanjing Medical University, Suzhou, China.
Department of Obstetrics and Gynecology, Suzhou Hospital, Affiliated Hospital of Medical School, Nanjing University, Suzhou, China.
出版信息
Front Cell Infect Microbiol. 2024 Aug 16;14:1405789. doi: 10.3389/fcimb.2024.1405789. eCollection 2024.
BACKGROUND
Vaginal microbiota is involved in human papillomavirus (HPV) infection and cervical cancer (CC) progression, and the specific changes in vaginal microbial composition during this process remains uncertain.
OBJECTIVE
This study aimed to observe the changes in the specific composition of vaginal microorganisms in different cervical lesions and identify biomarkers at different stages of lesions.
METHODS
In this study we used the illumina high-throughput gene sequencing technology to determine the V4 region of 16SrRNA and observed the vaginal microbial composition in different cervical lesions.
RESULTS
The vaginal microbiota of patients with high-risk HPV infection and cervical lesions is significantly different from that of the normal population, but there is no significant difference in the richness of vaginal microbes. The diversity of vaginal species in CC patients is higher than that in high-risk HPV infection or CIN patients. The main manifestation is an increase in the diversity of vaginal microbes, a decrease in the relative abundance of cyanobacteria and Lactobacillus, and an increase in the relative abundance of dialister, peptonephila and other miscellaneous bacteria. There are characteristic vaginal biomarker in normal women, high risk HPV patients and CC patients. In detail, the biomarker in the normal group was varibaculum, the biomarker in the high-risk HPV group was saccharopolyspora, the biomarker of the CC group was the Proteobacteria, Corynebacterium, Coprococcus, Peptococcus and Ruminococcus.
CONCLUSIONS
The study indicated that the compositions of vaginal microbes in different cervical lesions is different. The vaginal microbial composition has a certain diagnostic effect on healthy women, patients with high-risk HPV infection and cervical lesions. These microbes may serve as potential biomarkers for CC. It also provided an effective way for the treatment of HPV infections and cervical lesions.
背景
阴道微生物群参与人乳头瘤病毒（HPV）感染和宫颈癌（CC）的进展，在此过程中阴道微生物组成的具体变化尚不确定。
目的
本研究旨在观察不同宫颈病变中阴道微生物特定组成的变化，并鉴定病变不同阶段的生物标志物。
方法
本研究采用高通量基因测序技术检测 16SrRNA 的 V4 区，观察不同宫颈病变患者的阴道微生物组成。
结果
高危 HPV 感染和宫颈病变患者的阴道微生物群与正常人群明显不同，但阴道微生物的丰富度没有显著差异。CC 患者的阴道物种多样性高于高危 HPV 感染或 CIN 患者。主要表现为阴道微生物多样性增加，蓝细菌和乳杆菌相对丰度降低，Dialister、Peptonephila 等杂菌相对丰度增加。正常女性、高危 HPV 患者和 CC 患者存在特征性的阴道生物标志物。具体而言，正常组的标志物为 Varibaculum，高危 HPV 组的标志物为 Saccharopolyspora，CC 组的标志物为 Proteobacteria、Corynebacterium、Coprococcus、Peptococcus 和 Ruminococcus。
结论
本研究表明，不同宫颈病变患者的阴道微生物组成不同。阴道微生物组成对健康女性、高危 HPV 感染和宫颈病变患者具有一定的诊断作用。这些微生物可能是 CC 的潜在生物标志物。它还为 HPV 感染和宫颈病变的治疗提供了一种有效的方法。
Zotero 插件