Asadinezhad Maryam, Pakzad Iraj, Asadollahi Parisa, Ghafourian Sobhan, Kalani Behrooz Sadeghi
Students Research Committee, Ilam University of Medical Sciences, Ilam, Iran.
Department of Microbiology, Faculty of Medicine, Ilam University of Medical Sciences, Ilam, Iran.
Bioinform Biol Insights. 2024 Aug 30;18:11779322241272404. doi: 10.1177/11779322241272404. eCollection 2024.
Brucellosis is a chronic and debilitating disease in humans, causing great economic losses in the livestock industry. Making an effective vaccine is one of the most important concerns for this disease. The new mRNA vaccine technology due to its accuracy and high efficiency has given promising results in various diseases. The objective of this research was to create a novel mRNA vaccine with multiple epitopes targeting . Seventeen antigenic proteins and their appropriate epitopes were selected with immunoinformatic tools and surveyed in terms of toxicity, allergenicity, and homology. Then, their presentation and identification by MHC cells and other immune cells were checked with valid tools such as molecular docking, and a multi-epitope protein was modeled, and after optimization, mRNA was analyzed in terms of structure and stability. Ultimately, the immune system's reaction to this novel vaccine was evaluated and the results disclosed that the designed mRNA construct can be an effective and promising vaccine that requires laboratory and clinical trials.
布鲁氏菌病是一种人类慢性衰弱性疾病，给畜牧业造成巨大经济损失。研制有效的疫苗是应对这种疾病的最重要关注点之一。新型mRNA疫苗技术因其准确性和高效性，已在多种疾病中取得了有前景的成果。本研究的目的是创建一种针对……的具有多个表位的新型mRNA疫苗。利用免疫信息学工具选择了17种抗原蛋白及其合适的表位，并对其毒性、致敏性和同源性进行了评估。然后，使用分子对接等有效工具检查它们在MHC细胞和其他免疫细胞中的呈递和识别情况，对一种多表位蛋白进行建模，优化后，从结构和稳定性方面对mRNA进行分析。最终，评估了免疫系统对这种新型疫苗的反应，结果表明所设计的mRNA构建体可能是一种有效且有前景的疫苗，尚需进行实验室和临床试验。
