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基于数据驱动和细胞特异性的细胞核相关肌动蛋白结构测定

Data-Driven and Cell-Specific Determination of Nuclei-Associated Actin Structure.

作者信息

Nikitina Nina, Bursa Nurbanu, Goelzer Matthew, Goldfeldt Madison, Crandall Chase, Howard Sean, Rubin Janet, Zavala Anamaria, Satici Aykut, Uzer Gunes

机构信息

Boise State University.

University of Idaho.

出版信息

Small Struct. 2024 May;5(5). doi: 10.1002/sstr.202300204. Epub 2024 Feb 16.

DOI:10.1002/sstr.202300204
PMID:39220563
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC11361466/
Abstract

Quantitative and volumetric assessment of filamentous actin fibers (F-actin) remains challenging due to their interconnected nature, leading researchers to utilize threshold based or qualitative measurement methods with poor reproducibility. Here we introduce a novel machine learning based methodology for accurate quantification and reconstruction of nuclei-associated F-actin. Utilizing a Convolutional Neural Network (CNN), we segment actin filaments and nuclei from 3D confocal microscopy images and then reconstruct each fiber by connecting intersecting contours on cross-sectional slices. This allowed measurement of the total number of actin filaments and individual actin filament length and volume in a reproducible fashion. Focusing on the role of F-actin in supporting nucleocytoskeletal connectivity, we quantified apical F-actin, basal F-actin, and nuclear architecture in mesenchymal stem cells (MSCs) following the disruption of the Linker of Nucleoskeleton and Cytoskeleton (LINC) Complexes. Disabling LINC in mesenchymal stem cells (MSCs) generated F-actin disorganization at the nuclear envelope characterized by shorter length and volume of actin fibers contributing a less elongated nuclear shape. Our findings not only present a new tool for mechanobiology but introduce a novel pipeline for developing realistic computational models based on quantitative measures of F-actin.

摘要

由于丝状肌动蛋白纤维（F-肌动蛋白）相互连接的特性，对其进行定量和体积评估仍然具有挑战性，这导致研究人员采用基于阈值或定性的测量方法，而这些方法的可重复性较差。在此，我们引入了一种基于机器学习的新方法，用于准确量化和重建与细胞核相关的F-肌动蛋白。利用卷积神经网络（CNN），我们从三维共聚焦显微镜图像中分割出肌动蛋白丝和细胞核，然后通过连接横截面切片上的相交轮廓来重建每根纤维。这使得我们能够以可重复的方式测量肌动蛋白丝的总数、单个肌动蛋白丝的长度和体积。聚焦于F-肌动蛋白在支持核细胞骨架连接中的作用，我们在核骨架与细胞骨架连接复合体（LINC复合体）被破坏后，对间充质干细胞（MSC）中的顶端F-肌动蛋白、基底F-肌动蛋白和核结构进行了量化。在间充质干细胞（MSC）中使LINC失活会导致核膜处F-肌动蛋白紊乱，其特征是肌动蛋白纤维的长度和体积较短，导致核形状不太细长。我们的研究结果不仅为力学生物学提供了一种新工具，还引入了一种基于F-肌动蛋白定量测量来开发逼真计算模型的新流程。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f066/11361466/2797541cfa9c/nihms-1965343-f0007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f066/11361466/c1548ff78838/nihms-1965343-f0002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f066/11361466/1134d50c2c9c/nihms-1965343-f0003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f066/11361466/adc47aec2873/nihms-1965343-f0004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f066/11361466/117edde34210/nihms-1965343-f0005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f066/11361466/dff5376a6eb3/nihms-1965343-f0006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f066/11361466/2797541cfa9c/nihms-1965343-f0007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f066/11361466/c1548ff78838/nihms-1965343-f0002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f066/11361466/1134d50c2c9c/nihms-1965343-f0003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f066/11361466/adc47aec2873/nihms-1965343-f0004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f066/11361466/117edde34210/nihms-1965343-f0005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f066/11361466/dff5376a6eb3/nihms-1965343-f0006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f066/11361466/2797541cfa9c/nihms-1965343-f0007.jpg

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本文引用的文献

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2
CD93 Signaling via Rho Proteins Drives Cytoskeletal Remodeling in Spreading Endothelial Cells.CD93 通过 Rho 蛋白信号传导驱动扩展内皮细胞中的细胞骨架重塑。
Int J Mol Sci. 2021 Nov 17;22(22):12417. doi: 10.3390/ijms222212417.
3
The role of GPR56/ADGRG1 in health and disease.GPR56/ADGRG1 在健康和疾病中的作用。
Biomed J. 2021 Oct;44(5):534-547. doi: 10.1016/j.bj.2021.04.012. Epub 2021 May 4.
4
Fiber finding algorithm using stepwise tracing to identify biopolymer fibers in noisy 3D images.使用逐步跟踪的纤维发现算法来识别噪声 3D 图像中的生物聚合物纤维。
Biophys J. 2021 Sep 21;120(18):3860-3868. doi: 10.1016/j.bpj.2021.08.017. Epub 2021 Aug 17.
5
Lamin A/C Is Dispensable to Mechanical Repression of Adipogenesis.核纤层蛋白A/C对脂肪生成的机械抑制作用并非必需。
Int J Mol Sci. 2021 Jun 19;22(12):6580. doi: 10.3390/ijms22126580.
6
Automated and semi-automated enhancement, segmentation and tracing of cytoskeletal networks in microscopic images: A review.微观图像中细胞骨架网络的自动化和半自动增强、分割与追踪：综述
Comput Struct Biotechnol J. 2021 Apr 15;19:2106-2120. doi: 10.1016/j.csbj.2021.04.019. eCollection 2021.
7
The ImageJ ecosystem: Open-source software for image visualization, processing, and analysis.ImageJ 生态系统：用于图像可视化、处理和分析的开源软件。
Protein Sci. 2021 Jan;30(1):234-249. doi: 10.1002/pro.3993. Epub 2020 Nov 20.
8
Isolated nuclei stiffen in response to low intensity vibration.孤立核体会在受到低强度振动时变硬。
J Biomech. 2020 Oct 9;111:110012. doi: 10.1016/j.jbiomech.2020.110012. Epub 2020 Aug 28.
9
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Cells. 2020 Jan 6;9(1):132. doi: 10.3390/cells9010132.
10
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Biomech Model Mechanobiol. 2020 Jun;19(3):1155-1164. doi: 10.1007/s10237-019-01276-5. Epub 2019 Dec 14.