一种针对因子VIII相关抗原（VIIIRAg）的单克隆抗体在因子VIII免疫放射分析中的应用。
The application of a monoclonal antibody to factor VIII related antigen (VIIIRAg) in immunoradiometric assays for factor VIII.
作者信息
Thomas J E, Peake I R, Giddings J C, Welch A N, Bloom A L
出版信息
Thromb Haemost. 1985 Feb 18;53(1):143-7.
A two site solid phase immunoradiometric assay (IRMA) for VIIIRAg has been developed using a monoclonal antibody as both the solid phase ligand and the radiolabelled antibody. A range of normal plasmas and von Willebrand's disease (vWd) plasmas has been assayed for VIIIRAg by this method and compared with VIIIRAg values measured by an IRMA using polyclonal antibodies and by immunoelectrophoresis and with ristocetin cofactor activity (VIIIRiCoF). It was found that the monoclonal IRMA correlated well with the polyclonal IRMA and the immunoelectrophoretic assay, but the correlation with the VIIIRiCoF assay was relatively poor, in spite of the strong inhibitory effect of the antibody on VIIIRiCoF activity.
已开发出一种用于检测血管性血友病因子相关抗原（VIIIRAg）的双位点固相免疫放射分析（IRMA）方法，该方法使用一种单克隆抗体作为固相配体和放射性标记抗体。已用此方法检测了一系列正常血浆和血管性血友病（vWd）血浆中的VIIIRAg，并将结果与使用多克隆抗体的IRMA、免疫电泳法测得的VIIIRAg值以及瑞斯托霉素辅因子活性（VIIIRiCoF）进行了比较。结果发现，单克隆IRMA与多克隆IRMA及免疫电泳分析结果相关性良好，但尽管该抗体对VIIIRiCoF活性有很强的抑制作用，其与VIIIRiCoF分析的相关性相对较差。
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