凝血因子VIII促凝抗原的免疫放射测定法。
Immunoradiometric measurement of the factor VIII procoagulant antigen.
作者信息
Lazarchick J, Hoyer L W
出版信息
J Clin Invest. 1978 Nov;62(5):1048-52. doi: 10.1172/JCI109209.
Abstract
A fluid-phase immunoradiometric assay has been developed which identifies an antigen on the Factor VIII (antihemophilic factor) procoagulant protein. This sensitive and quantitative assay is not influenced by levels of Favor VIII-related antigen (von Willebrand factor) or other plasma proteins. There is a close correlation of procoagulant activity and immunologically detectable protein in normal and von Willebrand's disease plasmas. In contrast, several different patterns have been identified in hemophilic plasmas. Neither procoagulant activity nor procoagulant antigen is detectable in plasmas from patients with severe classic hemophilia. Patients with mild and moderate hemophilia have either comparable plasma concentrations of procoagulant activity and procoagulant antigen or relatively greater levels of immunologically detectable protein.
摘要
已开发出一种液相免疫放射分析方法，该方法可识别凝血因子VIII（抗血友病因子）促凝蛋白上的一种抗原。这种灵敏且定量的分析方法不受VIII因子相关抗原（血管性血友病因子）或其他血浆蛋白水平的影响。在正常血浆和血管性血友病血浆中，促凝活性与免疫可检测蛋白之间存在密切相关性。相比之下，在血友病血浆中已识别出几种不同模式。重度经典型血友病患者的血浆中既检测不到促凝活性，也检测不到促凝抗原。轻度和中度血友病患者的血浆中促凝活性和促凝抗原浓度相当，或者免疫可检测蛋白水平相对较高。
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