Laboratório de Genética Aplicada (LAGA), Instituto de Estudos Costeiros (IECOS), Universidade Federal do Pará, Bragança, Brazil.
Divisão de Agricultura, Instituto Superior Politécnico de Gaza, Chókwè, Mozambique.
PeerJ. 2024 Aug 28;12:e17581. doi: 10.7717/peerj.17581. eCollection 2024.
Ariidae species play a significant role as fishing resources in the Amazon region. However, the family's systematic classification is notably challenging, particularly regarding species delimitation within certain genera. This difficulty arises from pronounced morphological similarities among species, posing obstacles to accurate species recognition.
Following morphological identification, mitochondrial markers (COI and Cytb) were employed to assess the diversity of Ariidae species in the Amazon.
Our sampling efforts yielded 12 species, representing 92% of the coastal Amazon region's diversity. Morphological identification findings were largely corroborated by molecular data, particularly for species within the and genera. Nonetheless, despite morphological support, and displayed minimal genetic divergence (0.010). Similarly, and formed a single clade with no genetic divergence, indicating mitochondrial introgression. For the majority of taxa examined, both COI and Cytb demonstrated efficacy as DNA barcodes, with Cytb exhibiting greater polymorphism and resolution. Consequently, the molecular tools utilized proved highly effective for species discrimination and identification.
Ariidae 物种在亚马逊地区作为渔业资源发挥着重要作用。然而，该科的系统分类具有显著的挑战性，特别是在某些属内的物种划分方面。这种困难源于物种之间明显的形态相似性，给准确的物种识别带来了障碍。
在形态学鉴定之后，我们使用线粒体标记物（COI 和 Cytb）来评估亚马逊地区 Ariidae 物种的多样性。
我们的采样工作共获得了 12 个物种，代表了亚马逊沿海地区 92%的物种多样性。形态学鉴定结果在很大程度上得到了分子数据的支持，特别是在 和 属内的物种。然而，尽管有形态学的支持， 和 之间的遗传分化很小（0.010）。同样， 和 形成了一个没有遗传分化的单系群，表明存在线粒体基因渗入。对于大多数被研究的分类群来说，COI 和 Cytb 都可作为 DNA 条形码，Cytb 表现出更高的多态性和分辨率。因此，所使用的分子工具对于物种的区分和鉴定非常有效。
