Department of Transfusion Medicine, School of Laboratory Medicine and Biotechnology, Southern Medical University, Guangzhou 510515, PR China.
Department of Obstetrics, He Xian Memorial Affiliated Hospital of Southern Medical University, Guangzhou 511402, China.
Lab Chip. 2024 Sep 24;24(19):4639-4648. doi: 10.1039/d4lc00484a.
To report the testing signal of an immunochromatographic assay for on-site quantitative detection, a portable and user-friendly smartphone-based biosensing platform is developed in this study. This innovative system is composed of an ambient light sensor inherent smartphone reader and a 3D-printed handhold device, a quantitative tool capable of directly interpreting carbon nanoparticle (CNP)-conjugated immunochromatographic strips. To showcase the platform capability, the smartphone-based immunochromatography system (SPICS) reader and device were successfully used in CNP strips for rapid detection of the early pregnancy marker human chorionic gonadotropin in female urine (HCG; limit of detection [LOD]: 0.30 mIU mL), prostate-specific antigen in patient blood (PSA; LOD: 0.28 ng mL) and ampicillin residue in animal milk (AMP; LOD: 0.23 ng mL). The results were fully correlated with conventional commercial instruments ( = 0.99). The SPICS platform exhibits significant advantages, including portability, cost-effectiveness, easy operation, and rapid and quantitative detection, making it a valuable on-site diagnosis tool for use in home and community healthcare facilities.
为了报告免疫层析分析用于现场定量检测的测试信号，本研究开发了一种基于便携式、用户友好型智能手机的生物传感平台。该创新系统由智能手机内置的环境光传感器和 3D 打印的手持设备组成，是一种能够直接解读碳纳米粒子（CNP）标记免疫层析条的定量工具。为了展示该平台的能力，基于智能手机的免疫层析系统（SPICS）阅读器和设备成功用于快速检测女性尿液中的早期妊娠标志物人绒毛膜促性腺激素（HCG；检测限 [LOD]：0.30 mIU mL）、患者血液中的前列腺特异性抗原（PSA；LOD：0.28 ng mL）和动物乳中的氨苄西林残留（AMP；LOD：0.23 ng mL）。结果与传统商业仪器完全相关（= 0.99）。SPICS 平台具有便携、经济高效、操作简单以及快速定量检测等显著优势，使其成为家庭和社区医疗保健设施中进行现场诊断的有价值工具。
