He Haoting, Hao Dingji, Tian Linxiao, Zhu Congru, Guo Lili, Zhang Keao, Zhu Siyao
Department of Oncology, Tonglu County Hospital of Traditional Chinese Medicine, Tonglu County, Hangzhou City, Zhejiang Province.
Eur J Transl Myol. 2024 Aug 29;34(3):12678. doi: 10.4081/ejtm.2024.12678.
Papillary thyroid carcinoma is an epithelial malignancy with follicular cell differentiation and sets of defined nuclear features and appearance of an irregular solid mass. The main objective of our study is to research on the expression of miR-218-2 in the serum of patients with papillary thyroid cancer and its clinical significance. Our study involved patients with thyroid nodules were divided into a capitate cancer group (N = 100) and a benign nodule group (N =100). Lastly, 50 cases of healthy individuals were used as controls. The total sample size was 250. All cases were clinically diagnosed and underwent histopathological examinations at the Tonglu County Hospital of Traditional Chinese Medicine between January 2023 and January 2024. Quantitative RT-PCR was used to assess the expression levels of miR-218-2 and its host gene SLIT3 in normal and cancer thyroid tissues. We found that 45% of tumour sizes were less than 1 cm with 90% of tumours did not infiltrate the glandular capsule, implying a favourable prognosis. Lastly, 85% of tumours were well differentiated with about 75% showing no metastasis while 60% of TNM stage were classified as stage I. Also, miR-218-2 and its host gene SLIT3 are significantly down-regulated in papillary thyroid carcinoma. The inhibitory effects of miR-218-2 act in synergy with its host gene SLIT3 to alter the rates of cell invasion, cell migration and cell proliferation. Our findings have clinical significance on the involvement of miR-218-2 and SLIT3. There exists a functional relationship between host genes and intronic miRNAs in the tumorigenesis of thyroid cancers.
甲状腺乳头状癌是一种具有滤泡细胞分化的上皮性恶性肿瘤，具有明确的核特征和不规则实性肿块外观。本研究的主要目的是研究miR-218-2在甲状腺乳头状癌患者血清中的表达及其临床意义。我们的研究将甲状腺结节患者分为乳头状癌组（N = 100）和良性结节组（N = 100）。最后，将50例健康个体作为对照。总样本量为250例。所有病例均于2023年1月至2024年1月在桐庐县中医医院进行临床诊断并接受组织病理学检查。采用定量逆转录聚合酶链反应（qRT-PCR）评估miR-218-2及其宿主基因SLIT3在正常甲状腺组织和癌组织中的表达水平。我们发现45%的肿瘤大小小于1 cm，90%的肿瘤未侵犯腺包膜，提示预后良好。最后，85%的肿瘤分化良好，约75%无转移，60%的TNM分期为I期。此外，miR-218-2及其宿主基因SLIT3在甲状腺乳头状癌中显著下调。miR-218-2的抑制作用与其宿主基因SLIT3协同作用，改变细胞侵袭、迁移和增殖率。我们的研究结果对miR-218-2和SLIT3的参与具有临床意义。在甲状腺癌的发生过程中，宿主基因和内含子miRNA之间存在功能关系。
