Yang Yuanzhe, Song Jiajun, Shen Siyuan, Wang Yongsheng, Pan Chuanying, Wu Jiyao, Lan Xianyong
College of Animal Science and Technology, Northwest A&F University, Yangling, Shaanxi, China.
College of Veterinary Medicine, Northwest A&F University, Yangling, Shaanxi, China.
Anim Biotechnol. 2024 Nov;35(1):2397806. doi: 10.1080/10495398.2024.2397806. Epub 2024 Sep 2.
Functioning as a key regulator of circadian rhythms, the gene exerts a substantial impact on the reproductive traits of animals. However, the effect of the gene on ovarian development remains unclear. In order to examine the relationship between bovine reproductive trait and the gene, a total of 901 ovarian samples were collected, categorized into different oestrus cycles (proestrus, oestrus, post-oestrus, anoestrous), and subjected to analysis for two potential insertion/deletions (InDels) in the gene. Through agarose gel electrophoresis and DNA sequencing, two polymorphic deletion mutations (P2-D, P3-D) were identified. Furthermore, a significant association between mature follicle diameter and P2-D was found ( < 0.05). Additionally, several significant correlations with ovarian length, width, height, and white body diameter were found for P3-D ( < 0.05). These findings suggested that the bovine gene plays an important role in above-mentioned reproductive traits, offering new avenues for improving cow fertility through marker-assisted selection (MAS).
作为昼夜节律的关键调节因子，该基因对动物的繁殖性状产生重大影响。然而，该基因对卵巢发育的影响仍不清楚。为了研究牛繁殖性状与该基因之间的关系，共收集了901个卵巢样本，根据发情周期（发情前期、发情期、发情后期、乏情期）进行分类，并对该基因中的两个潜在插入/缺失（InDels）进行分析。通过琼脂糖凝胶电泳和DNA测序，鉴定出两个多态性缺失突变（P2-D、P3-D）。此外，发现成熟卵泡直径与P2-D之间存在显著关联（P < 0.05）。另外，发现P3-D与卵巢长度、宽度、高度和白体直径存在若干显著相关性（P < 0.05）。这些发现表明，牛的该基因在上述繁殖性状中发挥重要作用，为通过标记辅助选择（MAS）提高奶牛繁殖力提供了新途径。
