Pharmaceutical Analytical Chemistry Department, Faculty of Pharmacy, Tanta University, Tanta, Egypt.
Zeta Pharma for Pharmaceutical Industries, Sadat City, Egypt.
Luminescence. 2024 Sep;39(9):e4889. doi: 10.1002/bio.4889.
Based on novel phosphorus-doped carbon dots (PCDs), a simple, quick, and accurate fluorescence probe for sarecycline (SAR) determination has been created. The PCDs were prepared in just five minutes using green, straightforward one-step microwave pyrolysis. To create the PCD probe, sodium phosphate monobasic was utilized as a phosphorus dopant and citric acid as a carbon supply. The proposed synthesis method was energy efficient and yielded CDs with a narrow particle size distribution. Based on inner-filter effect mechanism, the generated PCDs were used as nano-probe for SAR determination. The fluorescence quenching intensity showed a strong linear relationship with SAR concentration in the 3-90-μM range with a detection limit of 0.88 μM. Because there is no surface alteration of the CDs or creation of a covalent bond between SAR and PCDs, the developed approach is quick, easy, inexpensive, and requires less time. The new probe's enhanced sensitivity, broad linear range, and acceptable selectivity made it suitable for SAR measurement in pharmaceutical formulations and spiked human plasma. Most importantly, the Green Analytical Procedure Index (GAPI) and Analytical GREEnness (AGREE) assessments showed that the suggested method was environmentally friendly.
基于新型磷掺杂碳点（PCD），我们成功开发了一种用于色霉素（SAR）测定的简单、快速、准确的荧光探针。PCD 的制备仅需 5 分钟，采用绿色、简便的一步微波热解法。该 PCD 探针以磷酸二氢钠作为磷掺杂剂，柠檬酸作为碳源。所提出的合成方法具有节能优势，且生成的 CDs 具有较窄的粒径分布。基于内滤效应机制，生成的 PCD 被用作 SAR 测定的纳米探针。荧光猝灭强度在 3-90 μM 范围内与 SAR 浓度呈强线性关系，检测限为 0.88 μM。由于 CDs 表面没有改变，SAR 和 PCD 之间也没有形成共价键，因此该方法快速、简单、廉价，所需时间较短。新探针具有更高的灵敏度、更宽的线性范围和可接受的选择性，适用于药物制剂和加标人血浆中 SAR 的测定。最重要的是，绿色分析程序指数（GAPI）和分析绿色性评估（AGREE）表明，该方法具有环境友好性。
