Department of Pharmaceutical Analytical Chemistry, Faculty of Pharmacy, Helwan University, Cairo, Egypt.
Luminescence. 2024 Oct;39(10):e4923. doi: 10.1002/bio.4923.
A rapid, green and sensitive technique for the determination of metformin determination was developed based on the direct fluorescence enhancement of carbon dots (CDs) induced by the cited drug. The water-soluble CDs were prepared via a one-pot synthesis from avocado peels using domestic microwave. The prepared CDs exhibited strong fluorescence at 405 nm after excitation at 320 nm with a quantum yield of 51%. The fluorescence of CDs was enhanced linearly by increasing the concentration of metformin within the range 0.5-25 μg/mL with limit of detection 0.087 μg/mL and limit of quantification 0.263 μg/mL. The designed probe was proved to be selective toward metformin in the presence of other drugs such as vildagliptin and alogliptin and also in the presence of excipients in the pharmaceutical dosage form. The suggested and reported methods were compared with the help of the whiteness and greenness tools, specifically the white analytical chemistry and analytical greenness metric tools, for assessing hazardous solvents and reagents used.
建立了一种基于被分析药物诱导的碳点（CDs）直接荧光增强的快速、绿色、灵敏的二甲双胍测定技术。采用国产微波从鳄梨皮一步合成了水溶性 CDs，其在 320nm 激发下于 405nm 处显示出强荧光，量子产率为 51%。CDs 的荧光随着二甲双胍浓度在 0.5-25μg/mL 范围内的增加呈线性增强，检出限为 0.087μg/mL，定量限为 0.263μg/mL。在存在其他药物（如维格列汀和阿格列汀）和药物制剂赋形剂的情况下，该探针被证明对二甲双胍具有选择性。所提出和报告的方法通过白度和绿色度工具（特别是白色分析化学和分析绿色度度量工具）进行了比较，用于评估所用危险溶剂和试剂。
