Nacer Sabrine, Nassik Saâdia, El Ftouhy Fatima Zahra, Derqaoui Sophia, Mouahid Mohamed, Lkhider Mustapha
Laboratory of Virology Oncology Biosciences Environment and New Energies Faculty of Science and Technology Mohammedia University Hassan II, Casablanca, Morocco.
Avian Pathology Unit Department of Veterinary Pathology and Public Health Hassan II Agronomic and Veterinary Institute, Rabat, Morocco.
Int J Food Sci. 2024 Aug 26;2024:2790180. doi: 10.1155/2024/2790180. eCollection 2024.
Foodborne epidemics have become a serious public health emergency worldwide. Foods of animal origin, in particular chicken meat, are considered to be potential vectors of pathogenic bacteria, particularly . This bacterium can be resistant in the form of methicillin-resistant (MRSA) or produce enterotoxins leading to food poisoning when ingested. This study is aimed at exploring the virulence genes in responsible for producing enterotoxins (staphylococcal enterotoxin [SE] A [sea] and SE E [see]) and determining the prevalence of MRSA in raw broiler meat in the Casa-Rabat region in Morocco. A quantitative (q) PCR (qPCR) assay, using specific primers for (nuc) confirmation and detection of enterotoxin genes (sea and see), as well as the methicillin-resistant gene (mecA), was employed. Our findings indicated that all tested strains were positively identified as . Among them, one isolate (1/54) tested positive for the see gene (1.85%), while none carried the sea gene. Furthermore, the mecA gene, indicative of MRSA, was present in 12/54 of the isolates (22.22%). The potential presence of MRSA in Moroccan poultry meat underscores a public health risk. Thus, stringent measures are imperative to curtail the contamination and proliferation of this bacterium during the slaughtering process, underscoring the importance of continuing research into the prevalence of MRSA colonization among poultry slaughterhouse personnel.
食源性疾病已成为全球范围内严重的突发公共卫生事件。动物源性食品，尤其是鸡肉，被认为是致病细菌的潜在传播媒介，特别是 。这种细菌可以以耐甲氧西林 （MRSA） 的形式存在耐药性，或者在摄入后产生导致食物中毒的肠毒素。本研究旨在探索 中负责产生肠毒素（葡萄球菌肠毒素 [SE] A [sea] 和 SE E [see]）的毒力基因，并确定摩洛哥卡萨 - 拉巴特地区生鸡胸肉中 MRSA 的流行情况。采用定量（q）PCR（qPCR）检测方法，使用针对 （nuc） 确认和检测肠毒素基因（sea 和 see）以及耐甲氧西林基因（mecA）的特异性引物。我们的研究结果表明，所有测试菌株均被阳性鉴定为 。其中，一株分离株（1/54）的 see 基因检测呈阳性（1.85%），而没有一株携带 sea 基因。此外，表明 MRSA 的 mecA 基因存在于 12/54 的分离株中（22.22%）。摩洛哥禽肉中 MRSA 的潜在存在凸显了公共卫生风险。因此，必须采取严格措施减少这种细菌在屠宰过程中的污染和增殖，这突出了持续研究家禽屠宰场人员中 MRSA 定植流行情况的重要性。
