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从零售鸡肉中分离出的 的发生和肠毒素基因谱。

Occurrence and enterotoxin gene profiles of isolated from retail chicken meat.

机构信息

Department of Livestock Products Technology, Veterinary College, Bangalore, India.

Department of Livestock Products Technology (Meat Science), Madras Veterinary College, Chennai, India.

出版信息

Food Sci Technol Int. 2021 Oct;27(7):619-625. doi: 10.1177/1082013220980204. Epub 2020 Dec 13.

DOI:10.1177/1082013220980204
PMID:33307778
Abstract

The present study was conducted to evaluate the prevalence and enterotoxin gene profiles of isolated from 120 chicken meat marketed in retail outlets of Chennai, India. It was observed that total of 120 meat samples collected from different retail outlets, 66.67% (80/120) of the samples were positive for the presence of based on biochemical characterization and species specific PCR based on thermonuclease gene (). Enterotoxin gene profiling of the isolates for 9 genes () revealed that 52.50% (42/80) of the isolates in the present study were enterotoxigenic harboring either one or more gene. It was evident that majority of the isolates harbored , followed by , , , and either alone or in combination. None of the isolates harbored , and either alone or in combination. The results of the study clearly indicated higher prevalence of enterotoxigenic in retail meat marketed in Chennai, India indicating the potential of retail chicken meat to act as vehicle for food borne intoxication and a major public health threat.

摘要

本研究旨在评估印度钦奈零售点销售的 120 份鸡肉样本中分离出的 的流行率和肠毒素基因谱。结果观察到,在从不同零售点采集的总共 120 份肉样本中,基于生化特征和基于热稳定核酸酶基因()的种特异性 PCR,有 66.67%(80/120)的样本呈阳性,表明存在 。对分离株的 9 个基因()的肠毒素基因谱分析显示,本研究中的 52.50%(42/80)分离株为产肠毒素株,携带一个或多个基因。结果表明,大多数分离株携带 ,其次是 、 、 、 和 ,单独或组合携带。没有分离株单独或组合携带 、 或 。研究结果清楚地表明,在印度钦奈销售的零售鸡肉中,产肠毒素的 的流行率较高,这表明零售鸡肉有可能成为食源性中毒的载体,对公众健康构成重大威胁。

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