From waste to resource: Effects of digested rotten potato supernatant on the growth, total biomass and nutrient composition of .

A study was carried out to evaluate the growth performance of , a green microalga, in three different concentrations of digested rotten potato supernatant (DRPS) for 16 days. was grown in 20 % (T), 40 % (T), and 60 % (T) of the DRPS and at the same time in Bold Basal Medium (BBM) as a control (T). A significantly highest cell density of was found in T (192.83 ± 1.75 × 10 cells mL) in comparison to T (136.83 ± 5.58 × 10 cells mL), and T (99.11 ± 5.38 × 10 cells mL) (p < 0.001 for all comparisons) while the cell density at T (192.83 ± 1.75 × 10 cells mL) and T (180.907 ± 4.58 × 10 cells mL) did not differ significantly (p = 0.227). Moreover, the mean daily division rate of was significantly higher in T (0.340 ± 0.001 divisions day) in comparison to other concentrations of DRPS (p < 0.001 for all comparisons). The maximum value of total biomass (1.07 ± 0.10 g L) was found in T which was statistically similar to those in T and T. In addition, there was no significant difference between the mean maximum values of chlorophyll- content and optical density of in T and T. The highest protein content of 42.67 ± 0.57 % was observed in T which was significantly higher than T (39.43 ± 1.67 %) (p = 0.027). It is also worth mentioning that there was no significant difference in the crude lipid content of the microalgae grown in T (10.06 ± 0.17 %) and T (9.88 ± 0.14 %) (p = 0.616). Hence, 20 % DRPS can be used as an alternative culture media of BBM for with a broad aim to accelerate the sustainable advancement of microalgal production.