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将存活剂量率依赖性用作动态放射生物学模型的理论判别标准

The Use of Survival Dose-Rate Dependencies as Theoretical Discrimination Criteria for Dynamic Radiobiological Models.

作者信息

Mingo Barba Sergio, Lobo-Cerna Fernando, Krawczyk Przemek M, Lattuada Marco, Füchslin Rudolf M, Petri-Fink Alke, Scheidegger Stephan

机构信息

School of Engineering, Zürich University of Applied Sciences (ZHAW), Winterthur, Switzerland.

Chemistry Department, University of Fribourg, Fribourg, Switzerland.

出版信息

Dose Response. 2024 Aug 30;22(3):15593258241279906. doi: 10.1177/15593258241279906. eCollection 2024 Jul-Sep.

DOI:10.1177/15593258241279906
PMID:39224699
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC11367615/
Abstract

INTRODUCTION

Cell repair dynamics are crucial in optimizing anti-cancer therapies. Various assays (eg, comet assay and γ-H2AX) assess post-radiation repair kinetics, but interpreting such data is challenging and model-based data analyses are required. However, ambiguities in parameter calibration remain an unsolved challenge. To address this, we propose combining survival dose-rate effects with computer simulations to gain knowledge about repair kinetics.

METHODS

After a literature review, theoretical discriminators based on common fractionation/dose-rate-related effects were defined to discard unrealistic model dynamics. The Multi-Hit Repair (MHR) model was calibrated with canine osteosarcoma Abrams cell line data to study the discriminators' efficacy in scenarios with limited survival data. Additionally, survival dose-rate-dependent data from the human SiHa cervical cancer cell line were used to illustrate the survival behavior at diverse dose-rates and the capability of the MHR to model these data.

RESULTS

SiHa data confirmed the validity of the proposed discriminators. The discriminators filtered 99% of parameter sets, improving the calibration of Abrams cells data. Furthermore, results from both cell lines may hint universal aspects of cellular repair.

CONCLUSIONS

Dose-rate theoretical discrimination criteria are an effective method to understand repair kinetics and improve radiobiological model calibration. Moreover, this methodology may be used to analyze diverse biological data using dynamic models .

摘要

引言

细胞修复动力学对于优化抗癌治疗至关重要。各种检测方法（如彗星试验和γ-H2AX）可评估辐射后的修复动力学，但解读此类数据具有挑战性，需要基于模型的数据分析。然而，参数校准中的模糊性仍是一个未解决的挑战。为解决这一问题，我们建议将存活剂量率效应与计算机模拟相结合，以获取有关修复动力学的知识。

方法

在文献综述之后，基于常见的分次/剂量率相关效应定义了理论判别器，以舍弃不现实的模型动力学。使用犬骨肉瘤艾布拉姆斯细胞系数据对多击修复（MHR）模型进行校准，以研究判别器在存活数据有限的情况下的有效性。此外，利用人SiHa宫颈癌细胞系的存活剂量率依赖性数据来说明不同剂量率下的存活行为以及MHR对这些数据进行建模的能力。

结果

SiHa数据证实了所提出判别器的有效性。判别器筛选出了99%的参数集，改进了艾布拉姆斯细胞数据的校准。此外，两种细胞系的结果可能暗示了细胞修复的普遍方面。

结论

剂量率理论判别标准是理解修复动力学和改进放射生物学模型校准的有效方法。此外，这种方法可用于使用动态模型分析各种生物学数据。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5654/11367615/723db28f4a5e/10.1177_15593258241279906-fig11.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5654/11367615/1586d016642e/10.1177_15593258241279906-fig1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5654/11367615/c536b5094d49/10.1177_15593258241279906-fig2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5654/11367615/d70e995d74f5/10.1177_15593258241279906-fig3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5654/11367615/6db586e16464/10.1177_15593258241279906-fig4.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5654/11367615/44f358c111b0/10.1177_15593258241279906-fig5.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5654/11367615/e9c12a7588c6/10.1177_15593258241279906-fig6.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5654/11367615/1209dea5707a/10.1177_15593258241279906-fig7.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5654/11367615/56a36c2103a6/10.1177_15593258241279906-fig8.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5654/11367615/9c4f314ff257/10.1177_15593258241279906-fig9.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5654/11367615/81bb235d3ec5/10.1177_15593258241279906-fig10.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5654/11367615/723db28f4a5e/10.1177_15593258241279906-fig11.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5654/11367615/1586d016642e/10.1177_15593258241279906-fig1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5654/11367615/c536b5094d49/10.1177_15593258241279906-fig2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5654/11367615/d70e995d74f5/10.1177_15593258241279906-fig3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5654/11367615/6db586e16464/10.1177_15593258241279906-fig4.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5654/11367615/44f358c111b0/10.1177_15593258241279906-fig5.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5654/11367615/e9c12a7588c6/10.1177_15593258241279906-fig6.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5654/11367615/1209dea5707a/10.1177_15593258241279906-fig7.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5654/11367615/56a36c2103a6/10.1177_15593258241279906-fig8.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5654/11367615/9c4f314ff257/10.1177_15593258241279906-fig9.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5654/11367615/81bb235d3ec5/10.1177_15593258241279906-fig10.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5654/11367615/723db28f4a5e/10.1177_15593258241279906-fig11.jpg

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本文引用的文献

1
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Phys Med Biol. 2022 Dec 19;68(1):015004. doi: 10.1088/1361-6560/aca5e7.
2
53BP1 Repair Kinetics for Prediction of In Vivo Radiation Susceptibility in 15 Mouse Strains.53BP1 修复动力学预测 15 种小鼠品系的体内辐射敏感性。
Radiat Res. 2020 Nov 10;194(5):485-499. doi: 10.1667/RADE-20-00122.1.
3
Holistic View on Cell Survival and DNA Damage: How Model-Based Data Analysis Supports Exploration of Dynamics in Biological Systems.
整体视角下的细胞存活与 DNA 损伤：基于模型的数据分析如何支持对生物系统动态的探索。
Comput Math Methods Med. 2020 Jul 6;2020:5972594. doi: 10.1155/2020/5972594. eCollection 2020.
4
The linear quadratic model: usage, interpretation and challenges.线性二次模型：使用、解释和挑战。
Phys Med Biol. 2018 Dec 19;64(1):01TR01. doi: 10.1088/1361-6560/aaf26a.
5
Both Complexity and Location of DNA Damage Contribute to Cellular Senescence Induced by Ionizing Radiation.DNA损伤的复杂性和位置均对电离辐射诱导的细胞衰老有影响。
PLoS One. 2016 May 17;11(5):e0155725. doi: 10.1371/journal.pone.0155725. eCollection 2016.
6
About the usefulness of biology-based models to describe cell survival after exposure to ionizing radiation.
Z Med Phys. 2015 Dec;25(4):309-10. doi: 10.1016/j.zemedi.2015.09.004.
7
Using Active Learning for Speeding up Calibration in Simulation Models.利用主动学习加速仿真模型中的校准
Med Decis Making. 2016 Jul;36(5):581-93. doi: 10.1177/0272989X15611359. Epub 2015 Oct 15.
8
DNA damage foci: Meaning and significance.DNA损伤灶：含义与意义。
Environ Mol Mutagen. 2015 Jul;56(6):491-504. doi: 10.1002/em.21944. Epub 2015 Mar 12.
9
Effect of varying dose-per-pulse and average dose rate in X-ray beam irradiation on cultured cell survival.X射线束照射中不同脉冲剂量和平均剂量率对培养细胞存活的影响。
Radiat Environ Biophys. 2014 Nov;53(4):671-6. doi: 10.1007/s00411-014-0565-2. Epub 2014 Aug 29.
10
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Comput Math Methods Med. 2013;2013:587543. doi: 10.1155/2013/587543. Epub 2013 Dec 16.