Pharmaceutical Analytical Chemistry Department, Faculty of Pharmacy, Cairo University, Kasr El-Aini Street, Cairo 11562, Egypt.
Anal Methods. 2024 Sep 26;16(37):6392-6402. doi: 10.1039/d4ay00914b.
Antibiotics are essential in treating infectious diseases in both humans and animals, and they are also utilized to enhance animal growth. However, their widespread use has led to significant environmental concerns. After administration of antibiotics, a substantial portion of them is excreted by animals, contaminating various environmental compartments. This problem is examined from the One Health perspective which seeks to balance human, animal, and environmental health for the benefit of global well-being. Enrofloxacin (ENR) is a commonly used antibiotic in veterinary medicine. Despite its efficacy in animal health, ENR is not approved for human use due to its associated toxicities. To address ENR detection, a sensor built upon a core-shell molecularly imprinted polymer (MIP) was created for the determination and testing of ENR in different matrices. Offering a miniaturized and reproducible tool for determining antibiotic residues in biological and environmental samples helps in revolutionizing the way we monitor and control antibiotic usage and contamination in various settings. The fabricated sensor demonstrated an optimum response time and functioned effectively across the pH range of 2.0 to 5.0. The potential profile displayed a linear correlation within a varying concentration spectrum of 1.0 × 10 M to 1.0 × 10 M characterized by a slope of 57.21 mV per decade. Furthermore, a comprehensive assessment of the environmental sustainability of the developed method was carried out using the Analytical Greenness calculator, AGREE algorithm. Lastly, an examination of the method's level of environmental friendliness was pursued using the newly developed RGB12 model to evaluate its "whiteness" level.
抗生素在治疗人类和动物的传染病方面至关重要，同时也被用于促进动物生长。然而，它们的广泛使用已经引起了重大的环境问题。抗生素在被动物使用后，其中很大一部分会被排泄出来，从而污染各种环境介质。这种问题从“One Health”的角度进行了研究，该角度旨在平衡人类、动物和环境的健康，以造福全球福祉。恩诺沙星（ENR）是兽医领域常用的抗生素。尽管它在动物健康方面具有疗效，但由于其毒性，ENR 并未被批准用于人类。为了解决 ENR 的检测问题，我们开发了一种基于核壳型分子印迹聚合物（MIP）的传感器，用于在不同基质中测定和测试 ENR。这种传感器为生物和环境样品中抗生素残留的测定提供了一种微型化和可重复使用的工具，有助于彻底改变我们在各种环境中监测和控制抗生素使用和污染的方式。所制备的传感器显示出最佳的响应时间，并且在 pH 值为 2.0 到 5.0 的范围内有效运行。该传感器的潜在特性显示出在 1.0×10 M 至 1.0×10 M 的浓度范围内呈线性相关，斜率为 57.21 mV/decade。此外，使用 Analytical Greenness 计算器和 AGREE 算法对所开发方法的环境可持续性进行了全面评估。最后，使用新开发的 RGB12 模型评估了该方法的环境友好性，以评估其“白度”水平。
