Tabassum Rukhsana, Dilshad Erum
Department of Bioinformatics and Biosciences, Faculty of Health and Life Sciences, Capital University of Science and Technology (CUST), Islamabad, Pakistan.
Drug Dev Ind Pharm. 2024 Sep 10:1-10. doi: 10.1080/03639045.2024.2400209.
The current research work focused on the evaluation of of and FeO NPs against liver cancer cell line (HepG2) by performing antiproliferative assay targeting the gene and apoptotic pathway genes and proteins.
FeO NPs were synthesized using extracts of and and characterized by UV-Vis spectroscopy, FTIR, SEM/EDS and XRD. MTT assay was used to study cytotoxicity against the HepG2 cells. Real-time qPCR and ELISA were used for the gene and protein analysis.
An absorbance peak at 300 nm for and 289 nm for nanoparticles were observed by UV-Vis analysis. The FTIR bands of FeO NPs suggested the presence of aldehydes, alcohols and polyols whereas bands of FeO NPs suggested the presence of carboxyl groups, hydroxyl groups, alkynes and amines. The size of FeO NPs was found to be 27 ± 5nm for and 84 ± 4nm for The IC value of 41.69 µM for and 71.04 µM for FeO NPs compared to plant extract (78.10 and 96.03 µM for and , respectively were found against HepG2 cells. The gene expression and protein levels of were decreased whereas those of , , and were found increased.
Nanoparticles and extract of were found more effective as compared to , which was evident by the results of cytotoxicity and analysis of studied genes and proteins.
当前的研究工作聚焦于通过针对 基因以及凋亡途径相关基因和蛋白质进行抗增殖试验，来评估 纳米颗粒和 纳米颗粒对肝癌细胞系（HepG2）的作用。
利用 提取物合成了 纳米颗粒，并通过紫外可见光谱、傅里叶变换红外光谱、扫描电子显微镜/能谱分析和X射线衍射对其进行表征。采用MTT法研究其对HepG2细胞的细胞毒性。运用实时定量聚合酶链反应和酶联免疫吸附测定法进行基因和蛋白质分析。
通过紫外可见分析观察到 纳米颗粒在300nm处有吸收峰， 纳米颗粒在289nm处有吸收峰。 纳米颗粒的傅里叶变换红外光谱带表明存在醛、醇和多元醇，而 纳米颗粒的光谱带表明存在羧基、羟基、炔烃和胺。发现 纳米颗粒的尺寸为27±5nm， 纳米颗粒的尺寸为84±4nm。与植物提取物相比， 纳米颗粒对HepG2细胞的半数抑制浓度值为41.69μM， 纳米颗粒为71.04μM（植物提取物对 和 的半数抑制浓度分别为78.10和96.03μM）。 的基因表达和蛋白质水平降低，而 、 、 和 的基因表达和蛋白质水平升高。
与 相比， 纳米颗粒和提取物被发现更有效，这在细胞毒性以及所研究基因和蛋白质的分析结果中得到了体现。
