Konappa Narasimhamurthy, Patil Rajeshwari H, Kariyappa Anupama S, Krishnamurthy Soumya, Ramachandrappa Niranjana Siddapura, Krishnappa Rahul, Chowdappa Srinivas
Department of Microbiology and Biotechnology, Jnanabharathi Campus, Bangalore University, Bengaluru, Karnataka 560 056 India.
Department of Microbiology, Field Marshal K. M. Cariappa College, A Constituent College of Mangalore University, Madikeri, Karnataka 571201 India.
Cytotechnology. 2025 Feb;77(1):16. doi: 10.1007/s10616-024-00674-7. Epub 2024 Dec 10.
The present study to production of silver nanoparticles (AgNPs) by leaf extracts of and to evaluate the activity of anticancer by using AgNPs against cancer cell lines such as MCF-7, HEPG2, H9C2, HEK293 and H1975. The synthesized AgNPs were characterized by using UV-Vis spectroscopy, EDS, FT-IR, XRD, DLS, SEM and HRTEM with SAED patterns. The surface plasmon resonance (SPR) of AgNPs formed a peak centered at 427 nm by UV-Vis analysis. FTIR analysis reveals that existence of functional groups subjected to silver ions reduction to metallic silver. Crystalline form of the AgNPs was assessed by XRD analysis, four spectral peaks at 111, 200, 220, and 311 were formed and zeta potential peak was found at 28.5 mV indicating the higher stability. The size average diameter of the AgNPs was between 27 and 30 nm by TEM and SEM analysis was reveals the morphology of AgNPs as elongated, irregular and aggregated and some particles are spherical. EDX analysis confirmed the elemental composition of AgNPs with 81.43% Ag. The average diameter of AgNPs was found 21.49 nm in diameter and width was about 12.01 nm by DLS analysis. Cytotoxicity of AgNPs was investigated by using MTT, SRB assay and comet assay was performed as a genotoxicity. The results revealed that AgNPs decreased the viability of cancer cells in a concentration dependent pattern (50 to 350 µg/ml). The influence of AgNPs on cell cycle stop was studied on H1975, HEP-G2 and MCF-7 cells and found that AgNPs could induce sub G0 cell cycle arrest. The AgNPs was also induced DNA fragmentation confirms the DNA damage in nanoparticles treated cell lines. The anticancer action of nanoparticles was analyzed using proapoptotic and antiapoptotic caspase 8 and caspase 3 mRNA expression levels. Finally the results suggested that AgNPs is an effective anticancer agent which induces apoptosis in H1975, HEP-G2 and MCF-7 cells. Based on our studies, further identification of the major compounds of leaf extracts is acceptable.
The online version contains supplementary material available at 10.1007/s10616-024-00674-7.
本研究旨在通过[植物名称]叶提取物制备银纳米颗粒（AgNPs），并评估AgNPs对MCF - 7、HEPG2、H9C2、HEK293和H1975等癌细胞系的抗癌活性。通过紫外可见光谱、能谱仪（EDS）、傅里叶变换红外光谱（FT - IR）、X射线衍射（XRD）、动态光散射（DLS）、扫描电子显微镜（SEM）和高分辨率透射电子显微镜（HRTEM）及选区电子衍射（SAED）图谱对合成的AgNPs进行表征。通过紫外可见分析，AgNPs的表面等离子体共振（SPR）在427 nm处形成一个峰值。傅里叶变换红外光谱分析表明存在使银离子还原为金属银的官能团。通过X射线衍射分析评估AgNPs的晶体形式，形成了111、200、220和311处的四个光谱峰，并且在28.5 mV处发现了zeta电位峰，表明具有较高的稳定性。通过透射电子显微镜（TEM）和扫描电子显微镜（SEM）分析，AgNPs的平均直径在27至30 nm之间，SEM分析揭示AgNPs的形态为细长、不规则且聚集的，一些颗粒为球形。能谱仪（EDX）分析证实AgNPs的元素组成为81.43%的银。通过动态光散射（DLS）分析，发现AgNPs的平均直径为21.49 nm，宽度约为12.01 nm。使用MTT法、磺酰罗丹明B（SRB）测定法研究AgNPs的细胞毒性，并进行彗星试验作为遗传毒性检测。结果表明，AgNPs以浓度依赖性模式（50至350μg/ml）降低癌细胞的活力。在H1975、HEP - G2和MCF - 7细胞上研究了AgNPs对细胞周期停滞的影响，发现AgNPs可诱导亚G0期细胞周期停滞。AgNPs还诱导DNA片段化，证实了纳米颗粒处理的细胞系中的DNA损伤。使用促凋亡和抗凋亡半胱天冬酶8和半胱天冬酶3的mRNA表达水平分析纳米颗粒的抗癌作用。最后结果表明，AgNPs是一种有效的抗癌剂，可诱导H1975、HEP - G2和MCF - 7细胞凋亡。基于我们的研究，进一步鉴定叶提取物的主要化合物是可行的。
在线版本包含可在10.1007/s10616 - 024 - 00674 - 7获取的补充材料。
