用于在鼠组织中进行内皮细胞的体积相关光 X 射线和电子显微镜术的方案。
Protocol for volume correlative light X-ray and electron microscopy of endothelial cells in mouse tissue.
机构信息
Centre for Microvascular Research, William Harvey Research Institute, Barts and the London School of Medicine and Dentistry, Queen Mary University of London, EC1M 6BQ London, UK; Departamento e Instituto de Biología Molecular (IUBM, UAM), Centro de Biología Molecular Severo Ochoa, Universidad Autónoma de Madrid, UAM-CSIC, Campus de Cantoblanco, 28049 Madrid, Spain.
Centre for Microvascular Research, William Harvey Research Institute, Barts and the London School of Medicine and Dentistry, Queen Mary University of London, EC1M 6BQ London, UK; Cancer Immunology and Immunotherapy Lab, Center for Cooperative Research in Biosciences (CIC BioGUNE), Basque Research and Technology Alliance (BRTA), Derio, 48160 Bizkaia, Spain.
出版信息
STAR Protoc. 2024 Sep 20;5(3):103257. doi: 10.1016/j.xpro.2024.103257. Epub 2024 Sep 1.
Correlative light and electron microscopy (CLEM) greatly facilitate capturing the ultrastructure of spatially and/or temporally rare events. Here, we present a protocol for targeting regions of interests (ROIs) in tissue endothelial cells (ECs) using X-ray micro-computed tomography (μCT). We describe steps for ROI targeting guided by vasculature patterns and positions of EC nuclei visualized by light and X-ray microscopy. The protocol is applicable to thin or translucent tissues that contain defined landmarks visible in both light and X-ray microscopy. For complete details on the use and execution of this protocol, please refer to Reglero-Real et al..
相关的光学和电子显微镜（CLEM）极大地促进了对空间和/或时间上罕见事件的超微结构的捕获。在这里，我们介绍了一种使用 X 射线微计算机断层扫描（μCT）靶向组织内皮细胞（EC）中感兴趣区域（ROI）的方案。我们描述了使用光和 X 射线显微镜可视化的血管模式和 EC 核位置来指导 ROI 靶向的步骤。该方案适用于包含在光和 X 射线显微镜中都可见的定义标记物的薄或半透明组织。有关此方案的使用和执行的完整详细信息，请参阅 Reglero-Real 等人的研究。
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