嗜热四膜虫中阿糖胞苷的代谢
Metabolism of cytosine arabinoside in Tetrahymena pyriformis.
作者信息
Benhura M A
出版信息
Comp Biochem Physiol B. 1985;80(4):821-5. doi: 10.1016/0305-0491(85)90468-7.
The metabolism of cytosine arabinoside (araC) in Tetrahymena pyriformis amicronucleate strain W was studied. araC inhibited cell multiplication and protein synthesis at concentrations higher than 0.1 and 0.25 M respectively. araC had no effect on protein synthesis. araC was converted to araCMP, araCDP and araCTP by homogenized cell preparations. A deaminase activity converted araC to uracil arabinoside. The deaminase activity totally inhibited by tetrahydrouridine (THU) at a concn of 4 X 10(-6) M. The Ki for THU was 8 X 10(-8) M.
研究了嗜热四膜虫无小核菌株W中阿糖胞苷（araC）的代谢。araC分别在浓度高于0.1和0.25M时抑制细胞增殖和蛋白质合成。araC对蛋白质合成无影响。araC被匀浆细胞制剂转化为araCMP、araCDP和araCTP。一种脱氨酶活性将araC转化为阿糖尿苷。该脱氨酶活性在浓度为4×10^(-6)M时被四氢尿苷（THU）完全抑制。THU的Ki为8×10^(-8)M。
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