Academy of Food Interdisciplinary Science, School of Food Science and Technology, Dalian Polytechnic University, Qinggongyuan1, Ganjingzi District, Dalian 116034, Liaoning, China; National Engineering Research Center of Seafood, Dalian Polytechnic University, Dalian 116034, Liaoning, China; Collaborative Innovation Center of Seafood Deep Processing, Dalian Polytechnic University, Dalian 116034, Liaoning, China; State Key Laboratory of Marine Food Processing and Safety Control, Dalian Polytechnic University, Dalian 116034, Liaoning, China; College of Food Science and Engineering, Jilin Agricultural University, Changchun 130118, Jilin, China.
Academy of Food Interdisciplinary Science, School of Food Science and Technology, Dalian Polytechnic University, Qinggongyuan1, Ganjingzi District, Dalian 116034, Liaoning, China; National Engineering Research Center of Seafood, Dalian Polytechnic University, Dalian 116034, Liaoning, China; Collaborative Innovation Center of Seafood Deep Processing, Dalian Polytechnic University, Dalian 116034, Liaoning, China; State Key Laboratory of Marine Food Processing and Safety Control, Dalian Polytechnic University, Dalian 116034, Liaoning, China.
Carbohydr Polym. 2024 Dec 1;345:122597. doi: 10.1016/j.carbpol.2024.122597. Epub 2024 Aug 9.
Fucoxanthin (Fx) has garnered significant interest due to its exceptional biological properties. However, its efficacy in enhancing food quality and human health is contingent upon the solubility of the compound in water and its physicochemical stability. Therefore, nanocarriers must be developed to enhance the stability and biocompatibility of Fx. In this study, oxidized paramylon and Fx self-assembled nanoparticles (Fx-OEP) were prepared via the anti-solvent method, with a loading rate of 82.47 % for Fx. The Fx-OEP exhibited robust storage and photostability. In vitro simulated digestion assays demonstrated that Fx-OEP effectively protected Fx from premature gastric release, while achieving a release efficiency of 72.17 % in the intestinal phase. Fx-OEP has the capacity to scavenge a range of reactive oxygen species (ROS) induced by cellular oxidative stress. Treatment with Fx-OEP resulted in a significant reduction in ROS accumulation in insulin-resistant HepG2 cells, which was attributed to the activation of the nuclear factor E2-related factor 2/heme oxygenase-1 (Nrf2/HO-1) pathway. This, in turn, activated insulin receptor substrate 1/glucose transporter type 4 (IRS1/GLUT4), promoting cellular glucose absorption and utilization. These findings indicate the potential of self-assembled nanoparticles based on oxidized paramylon as a new type of nanocarrier for delivering hydrophobic substances.
褐藻多酚（Fx）因其独特的生物学特性而备受关注。然而，其在提高食品质量和人类健康方面的功效取决于该化合物在水中的溶解度及其物理化学稳定性。因此，必须开发纳米载体来提高 Fx 的稳定性和生物相容性。在这项研究中，通过抗溶剂法制备了氧化原(paramylon)和 Fx 自组装纳米颗粒（Fx-OEP），Fx 的负载率为 82.47%。Fx-OEP 表现出良好的储存稳定性和光稳定性。体外模拟消化试验表明，Fx-OEP 能有效防止 Fx 在胃内过早释放，而在肠内阶段的释放效率达到 72.17%。Fx-OEP 能够清除细胞氧化应激诱导的多种活性氧（ROS）。用 Fx-OEP 处理可显著减少胰岛素抵抗 HepG2 细胞中 ROS 的积累，这归因于核因子 E2 相关因子 2/血红素加氧酶-1（Nrf2/HO-1）通路的激活。这反过来又激活了胰岛素受体底物 1/葡萄糖转运蛋白 4（IRS1/GLUT4），促进细胞对葡萄糖的吸收和利用。这些发现表明，基于氧化原的自组装纳米颗粒作为一种新型纳米载体，具有传递疏水性物质的潜力。
