Biotechnology Research Center and Department of Biotechnology, Toyama Prefectural University 5180 Kurokawa, Imizu, Toyama, 939-0398, Japan.
J Antibiot (Tokyo). 2024 Dec;77(12):847-851. doi: 10.1038/s41429-024-00770-y. Epub 2024 Sep 3.
Two new spirotetronate class compounds designated wychimicins E (1) and F (2) were isolated from the culture extract of an actinomycete Cryptosporangium sp. RD061707. Their structures were determined through extensive NMR analysis in comparison with wychimicin C. Both compounds lack one hydroxy group in the decalin moiety, and the relative configuration of the remaining hydroxy group was assigned by NMR analysis of triacetylwychimicin E (3). Compounds 1 and 2 showed potent antimicrobial activity against Kocuria rhizophila and Staphylococcus aureus. These compounds were also modestly cytotoxic against P388 murine leukemia cells.
从放线菌 Cryptosporangium sp. RD061707 的培养提取物中分离得到两种新的螺缩酮类化合物，命名为 wychimicins E（1）和 F（2）。通过与 wychimicin C 的广泛 NMR 分析比较，确定了它们的结构。这两种化合物在 decalin 部分都缺少一个羟基，其余羟基的相对构型通过三乙酰基 wychimicin E（3）的 NMR 分析确定。化合物 1 和 2 对腐皮镰刀菌和金黄色葡萄球菌表现出强烈的抗菌活性。这些化合物对 P388 小鼠白血病细胞也有一定的细胞毒性。
