Tropical Crops Genetic Resources Institute, Chinese Academy of Tropical Agricultural Sciences, Haikou, China.
National Key Laboratory for Tropical Crop Breeding, Sanya, China.
Physiol Plant. 2024 Sep-Oct;176(5):e14488. doi: 10.1111/ppl.14488.
As a commonly used medicinal plant, the flavonoid metabolites of Blumea balsamifera and their association with genes are still elusive. In this study, the total flavonoid content (TFC), flavonoid metabolites and biosynthetic gene expression patterns of B. balsamifera after application of exogenous methyl jasmonate (MeJA) were scrutinized. The different concentrations of exogenous MeJA increased the TFC of B. balsamifera leaves after 48 h of exposure, and there was a positive correlation between TFC and the elicitor concentration. A total of 48 flavonoid metabolites, falling into 10 structural classes, were identified, among which flavones and flavanones were predominant. After screening candidate genes by transcriptome mining, the comprehensive analysis of gene expression level and TFC suggested that FLS and MYB may be key genes that regulate the TFC in B. balsamifera leaves under exogenous MeJA treatment. This study lays a foundation for elucidating flavonoids of B. balsamifera, and navigates the breeding of flavonoid-rich B. balsamifera varieties.
作为一种常用的药用植物，艾纳香的类黄酮代谢物及其与基因的关联仍不明确。本研究探讨了外源茉莉酸甲酯（MeJA）处理后艾纳香总黄酮含量（TFC）、类黄酮代谢物和生物合成基因表达模式。不同浓度的外源 MeJA 处理后，艾纳香叶片的 TFC 在 48 小时后增加，TFC 与诱导剂浓度呈正相关。共鉴定出 48 种类黄酮代谢物，分为 10 种结构类型，其中以黄酮类和黄烷酮类为主。通过转录组挖掘筛选候选基因后，基因表达水平和 TFC 的综合分析表明，FLS 和 MYB 可能是调控外源 MeJA 处理下艾纳香叶片 TFC 的关键基因。本研究为阐明艾纳香的类黄酮奠定了基础，并为培育富含类黄酮的艾纳香品种提供了指导。
