Kiehle C P, Schubiger G
Dev Biol. 1985 Jun;109(2):336-46. doi: 10.1016/0012-1606(85)90460-9.
Upon fragmentation of a leg imaginal disc, cells near parts of the wounded surface are reprogrammed and form a blastema. This occurs without a change in fate and without the direct contact of the two wounded surfaces (G. H. Karpen and G. Schubiger, Nature (London) 294, 744-747, 1981). Two phases of the cell cycle have now been analyzed for several areas of disc fragments prior to and during wound healing. A mitotic index was used to compare the location of cell division, and autoradiography was used to reveal patterns of DNA synthesis. In contrast to the uniform division pattern in noncultured fragments, more dividing cells were observed near the two wound surfaces after 1 day of in vivo culture. During the second day, wound healing began and mitotic activity increased dramatically near both wound areas, and decreased in distant areas. Three and a half days of culture led to more complete wound closure and only cells on one site continued to show the highest frequency of labeled cells. It is concluded that changes in patterns of DNA synthesis and an increase in cell division begin prior to wound closure. This proliferation is consistent with the morphological changes and regulative behavior observed. In addition, the role of compartmental identity during regulation was tested. After wound closure began an increase in mitotic activity near wounds in the anterior compartment was observed whereas such an increase in division level was not seen in posterior cells near a wound.
腿部成虫盘破碎后，伤口表面附近的细胞会重新编程并形成芽基。这一过程中细胞命运没有改变，且两个伤口表面没有直接接触（G. H. 卡彭和G. 舒比格，《自然》（伦敦）294, 744 - 747, 1981）。现在，在伤口愈合之前和期间，对盘状碎片的几个区域的细胞周期的两个阶段进行了分析。使用有丝分裂指数来比较细胞分裂的位置，并使用放射自显影来揭示DNA合成模式。与未培养碎片中均匀的分裂模式不同，体内培养1天后，在两个伤口表面附近观察到更多的分裂细胞。在第二天，伤口愈合开始，两个伤口区域附近的有丝分裂活性急剧增加，而远处区域的有丝分裂活性下降。培养三天半后伤口更完全闭合，只有一个部位的细胞继续显示出最高频率的标记细胞。得出的结论是，DNA合成模式的变化和细胞分裂的增加在伤口闭合之前就开始了。这种增殖与观察到的形态变化和调节行为一致。此外，还测试了分区身份在调节过程中的作用。伤口闭合开始后，观察到前区伤口附近的有丝分裂活性增加，而伤口附近后区细胞的分裂水平没有这种增加。
