Schultz J E, Gotto J W, Weaver P F, Yoch D C
J Bacteriol. 1985 Jun;162(3):1322-4. doi: 10.1128/jb.162.3.1322-1324.1985.
Rhodospirillum rubrum was shown to grow fermentatively on fructose with N2 as a nitrogen source. The nitrogenase activity of these cells was regulated by the NH4+ switch-off/switch-on mechanism in a manner identical to that for photosynthetically grown cells. In vitro, the inactive nitrogenase Fe protein from fermenting cells was reactivated by an endogenous membrane-bound, Mn2+-dependent activating enzyme that was interchangeable with the activating enzyme isolated from photosynthetic membranes.
人们发现,深红红螺菌能在以N2作为氮源的条件下利用果糖进行发酵生长。这些细胞的固氮酶活性通过NH4+的关闭/开启机制进行调节,其方式与光合生长的细胞相同。在体外,来自发酵细胞的无活性固氮酶铁蛋白可被一种内源性膜结合的、依赖Mn2+的激活酶重新激活,该激活酶与从光合膜中分离出的激活酶可互换。