Biškauskaitė Renata, Lee Wen-Chien, Valeika Virgilijus
Kaunas University of Technology, Department of Polymer Chemistry and Technology, Radvilėnų pl. 19, Kaunas 50254, Lithuania.
National Chung Cheng University, Department of Chemical Engineering, No.168, Sec. 1, University Rd., Min-Hsiung Township, Chia-yi County 621301 R.O.C, Taiwan.
Heliyon. 2024 Aug 6;10(16):e35842. doi: 10.1016/j.heliyon.2024.e35842. eCollection 2024 Aug 30.
Enzymes are biological catalysts that exist in all living organisms. Proteases are one of the most important enzymes in the industry; microbial proteases are widely used in the food, textile, detergent, and leather industries. Traditionally, alkaline proteases are used in the leather industry for bating, however, due to environmental issues, there are many investigations for the application of proteolytic enzymes in other operations such as soaking, unhairing, and derma opening up. This study aimed to produce a proteolytic enzyme from BCRC 910501 and apply it to the soaking of salted hide. After cultivation, the crude enzyme was used for further analysis and leather processing. The results showed an enzyme with higher caseinolytic activity compared to keratinolytic activity. A soaking study found that enzyme improves rehydration, non-collagenous protein, and salt removal. Enzymatic soaking affected collagenous proteins more than the control, but did not significantly impact wet-blue leather properties. Using this enzyme in leather processing might be suitable to reduce the duration of soaking and the number of process steps.
酶是存在于所有活生物体中的生物催化剂。蛋白酶是该行业中最重要的酶之一；微生物蛋白酶广泛应用于食品、纺织、洗涤剂和皮革行业。传统上，碱性蛋白酶用于皮革工业中的软化处理，然而，由于环境问题，人们对蛋白水解酶在其他操作（如浸泡、脱毛和皮张开片）中的应用进行了许多研究。本研究旨在从 BCRC 910501 中生产一种蛋白水解酶，并将其应用于盐腌生皮的浸泡。培养后，粗酶用于进一步分析和皮革加工。结果表明，该酶的酪蛋白分解活性高于角蛋白分解活性。一项浸泡研究发现，该酶可改善复水、去除非胶原蛋白和盐分。酶法浸泡对角质蛋白的影响大于对照，但对蓝湿革性能没有显著影响。在皮革加工中使用这种酶可能适合减少浸泡时间和工艺步骤数量。
