Kumari Arpana, Kaur Balwinder, Srivastava Rajendra, Sangwan Rajender S
Centre of Innovative and Applied Bioprocessing, C-127, Phase-VIII, Industrial Area, SAS Nagar, Mohali 160071, Punjab, India.
Indian Institute of Technology Ropar, Nangal Road, Ropar 140001 Punjab, India.
Biochem Biophys Rep. 2015 May 29;2:108-114. doi: 10.1016/j.bbrep.2015.05.009. eCollection 2015 Jul.
Alkaline protease from brinjal leaf () having milk clotting activity has been purified to 9.44 fold to a final specific activity of 45.71 U/mg. SDS-PAGE of the final preparation revealed a single protein band of approx 14 kDa. Purified enzyme was characterized and was successfully immobilized into the amorphous mesoporous silica (SBA-15) and crystalline mesoporous zeolite (Nano-ZSM-5) using entrapment method. Maximum immobilization of 63.5% and 79.77% was obtained with SBA-15 and Nano-ZSM-5, respectively. This protocol serves as a novel approach for bioprocesses, mainly as milk coagulant for local dairy products and particularly, cheese making, and opens the new dimension of further research and other innovation.
具有凝乳活性的茄子叶碱性蛋白酶已被纯化至9.44倍，最终比活性为45.71 U/mg。最终制剂的SDS-PAGE显示出一条约14 kDa的单一蛋白带。对纯化的酶进行了表征，并使用包埋法成功地固定在无定形介孔二氧化硅（SBA-15）和结晶介孔沸石（纳米ZSM-5）中。SBA-15和纳米ZSM-5的最大固定化率分别为63.5%和79.77%。该方案是生物工艺的一种新方法，主要用作当地乳制品的凝乳剂，特别是用于制作奶酪，并为进一步研究和其他创新开辟了新的维度。
