1Department of Anesthesia, Medical Technical Institute, Southern Technical University, Basrah, Iraq.
2Pharmaceutical Chemistry Department, College of Pharmacy, University of Basrah, Iraq.
Georgian Med News. 2024 Jun(351):61-64.
The aim of the present investigation is to study HPLC process to evaluate Some Active Flavonoids in Ethyl Acetate Extract of Leaves of Butea monosperma Linn.
Using a soxhlation device, the leaves of Butea monosperma Linn. were extracted in stages. Each powdered batch (500g) was extracted in stages with polarity-graded solvents such as petroleum ether (Pet. Et) (60-80º), chloroform (CHCl3), ethyl acetate (EtOAc) using a soxhlet extractor. Alkaloids, flavonoids, glycosides, tannins, phenols, and steroids, among other chemical families of components, were identified through qualitative phytochemical screenings of each extract. To make a 10 g/ml stock, standard phenolic markers like quercetin, rutin, catechin, gallic acid, and chlorogenic acid were dissolved in methanol. Phytoconstituents were separated and identified from extracts using various solvents and combinations of solvents, which were chosen after consulting the literature.
Preliminary phytochemical screening showed the revealed that the leaves contain steroid, triterpenoids, fatty acid and alkaloids. While the ethyl acetate extract found to contain therapeutically important phytoconstitutes such as steroids, triterpenoids, saponins, flavonoids, and tannins. Bioactive extracts of Butea monosperma were found to include flavonoids and phenolic substances. In ethyl acetate extract, various flavonoids and phenolic compounds were discovered.
This is a preliminary report on the identification of phytochemical and HPLC evaluation of ethyl acetate extract of leaves of Butea monosperma Linn. and to unravel the mechanisms driving bioactive qualities and the existence of putative synergy among these substances, more research is needed on the isolation and characterization of individual Flavonoids or phenolic compounds.
本研究旨在利用 HPLC 方法对白花羊蹄甲叶的乙酸乙酯提取物中的一些活性类黄酮进行评估。
使用索氏提取器，对白花羊蹄甲叶进行分段提取。将每个粉末批次（500g）用极性梯度溶剂如石油醚（Pet. Et）（60-80º）、氯仿（CHCl3）、乙酸乙酯（EtOAc）分段提取。通过对每种提取物进行定性植物化学成分筛选，鉴定了生物碱、类黄酮、糖苷、单宁、酚类和类固醇等其他化学成分族。为了制备 10 g/ml 的储备液，将标准酚类标志物如槲皮素、芦丁、儿茶素、没食子酸和绿原酸溶解在甲醇中。从提取物中分离和鉴定植物成分，使用各种溶剂和溶剂组合，这些溶剂是在查阅文献后选择的。
初步的植物化学成分筛选表明，该叶含有甾体、三萜类、脂肪酸和生物碱。而乙酸乙酯提取物中发现含有甾体、三萜类、皂苷、类黄酮和单宁等具有治疗意义的植物成分。白花羊蹄甲的生物活性提取物包括类黄酮和酚类物质。在乙酸乙酯提取物中，发现了各种类黄酮和酚类化合物。
这是对白花羊蹄甲叶乙酸乙酯提取物的植物化学成分鉴定和 HPLC 评价的初步报告，并揭示了驱动生物活性品质的机制以及这些物质之间存在的潜在协同作用，需要进一步研究分离和鉴定单个类黄酮或酚类化合物。
