Strassmann G, Springer T A, Adams D O
J Immunol. 1985 Jul;135(1):147-51.
Macrophages activated and primed in vivo, although not resident or responsive macrophages, express the lymphocyte function associated (LFA)-1 antigen. By contrast, the biochemically related Mac-1 antigen is expressed on all populations of macrophages. In the present paper, we studied regulation of the LFA-1 antigen in vitro. LFA-1 could be induced in vitro on thioglycollate (TG)-elicited but not on proteose peptone (PP)-elicited or resident macrophages. Specifically, macrophage-activating factor (MAF), interferon-gamma (IFN-gamma), or picogram amounts of endotoxin (LPS) induced LFA-1 on TG-elicited macrophages following overnight incubation. Interferon, -alpha or -beta, fucoidin, and colony-stimulating factor were not effective. While some levels of LFA-1 could be detected as soon as 10 hr, peak expression was observed after 16 to 32 hr of incubation. The induction could be completely abrogated by cycloheximide, suggesting that protein synthesis was required. These results indicate that the induction of LFA-1 on mononuclear phagocytes is closely regulated and that the requirements for such induction are distinct from but share certain similarities with induction of cytotoxic functions and expression of Ia antigen.
在体内被激活和致敏的巨噬细胞，虽然不是驻留或反应性巨噬细胞，但表达淋巴细胞功能相关（LFA）-1抗原。相比之下，生化相关的Mac-1抗原在所有巨噬细胞群体上均有表达。在本文中，我们研究了体外LFA-1抗原的调节。LFA-1可在体外由巯基乙酸盐（TG）诱导的巨噬细胞上诱导产生，但在蛋白胨（PP）诱导的或驻留巨噬细胞上则不能诱导产生。具体而言，巨噬细胞激活因子（MAF）、干扰素-γ（IFN-γ）或皮克量的内毒素（LPS）在过夜孵育后可在TG诱导的巨噬细胞上诱导LFA-1产生。干扰素-α或-β、岩藻依聚糖和集落刺激因子无效。虽然在孵育10小时后就能检测到一定水平的LFA-1，但在孵育16至32小时后观察到峰值表达。该诱导可被放线菌酮完全消除，表明需要蛋白质合成。这些结果表明，单核吞噬细胞上LFA-1的诱导受到严格调控，并且这种诱导的要求与细胞毒性功能的诱导和Ia抗原的表达不同，但有一定相似之处。
