State Key Laboratory of Digital Medical Engineering, School of Biological Science and Medical Engineering, Southeast University, Nanjing 210096, China.
Langmuir. 2024 Sep 17;40(37):19517-19527. doi: 10.1021/acs.langmuir.4c01947. Epub 2024 Sep 4.
Colloidal crystal nanomaterials have been proven to be valuable substrates for optical-based biosensing due to their ordered macroporous nanostructure and brilliant optical properties. In this work, silica colloidal crystal (SCC) thin films, as well as polystyrene-SCC composite films and inverse opal (IO) polystyrene films fabricated using SCC as templates, are investigated for their application as substrate materials in optical interferometric biosensors. The SCC films formed by the self-assembly of silica colloidal crystals have the most densely packed nano-3D structure, also known as the opal structure. IO films are fabricated by filling the opal pores of SCC with polystyrene and then removing the template, resulting in an interconnected nano-3D ordered macroporous structure, as indicated by the name inverse opal. The performance of the three materials was compared and discussed based on an ordered porous layer interferometry optical platform, focusing on refractive index response, protein adsorption response, and biomolecular interaction response. These results could potentially offer innovative material support for the advancement of label-free optical biosensors, which can be used for more biological/biochemical/biomolecular reaction monitoring studies.
胶体晶体纳米材料由于其有序的大孔纳米结构和出色的光学性能，已被证明是基于光学的生物传感的有价值的基底。在这项工作中，研究了二氧化硅胶体晶体 (SCC) 薄膜以及使用 SCC 作为模板制备的聚苯乙烯-SCC 复合膜和反蛋白石 (IO) 聚苯乙烯膜，以将其作为光学干涉生物传感器中的基底材料。由二氧化硅胶体晶体自组装形成的 SCC 薄膜具有最密集的纳米 3D 结构，也称为蛋白石结构。IO 薄膜是通过用聚苯乙烯填充 SCC 的蛋白石孔然后去除模板来制备的，从而形成相互连接的纳米 3D 有序大孔结构，这就是反蛋白石的名称所表示的。基于有序多孔层干涉测量光学平台，对三种材料的性能进行了比较和讨论，重点关注折射率响应、蛋白质吸附响应和生物分子相互作用响应。这些结果可能为无标记光学生物传感器的发展提供创新的材料支持，可用于更多的生物/生化/生物分子反应监测研究。
