Cubells-Gómez Aitor, Lucío María Isabel, Bañuls María-José, Maquieira Ángel
Instituto Interuniversitario de Investigación de Reconocimiento Molecular y Desarrollo Tecnológico (IDM), Universitat Politècnica de València, Universitat de València, Camino de Vera s/n, 46022 Valencia, Spain.
Departamento de Química, Universitat Politècnica de València, Camino de Vera s/n, 46022 Valencia, Spain.
Gels. 2025 May 30;11(6):415. doi: 10.3390/gels11060415.
The creation of surface relief gratings using hydrogels for label-free biomolecule detection represents a significant advance in the development of versatile, cutting-edge biosensors. Central to this innovation is the formulation of materials with enhanced mechanical properties, especially for applications in soft, wearable technologies. In this work, we have developed novel biofunctional hydrogels that incorporate slide-ring supramolecular structures into their network, enabling the production of surface relief gratings with superior mechanical characteristics for biomolecule detection without the need for labels. These hydrogels, functionalized with bovine serum albumin and goat anti-rabbit antibodies, demonstrated excellent selectivity and sensitivity toward anti-bovine serum albumin and rabbit IgGs in blood serum, evaluated using a label-free format. Remarkably, the new materials matched the analytical performance of conventional hydrogels based on static networks while offering dramatically improved toughness and elasticity, with a compressive modulus comparable to human skin. This demonstrates the potential of slide-ring hydrogels for fabricating robust, label-free biosensing platforms. Furthermore, the flexibility of this system allows for the incorporation of various recognition elements tailored to specific applications.
利用水凝胶创建表面起伏光栅用于无标记生物分子检测，代表了多功能、前沿生物传感器开发的重大进展。这一创新的核心是配制具有增强机械性能的材料，特别是在软质可穿戴技术中的应用。在这项工作中，我们开发了新型生物功能水凝胶，其网络中纳入了滑环超分子结构，能够生产具有卓越机械特性的表面起伏光栅用于生物分子检测，无需标记。这些用水牛血清白蛋白和山羊抗兔抗体功能化的水凝胶，在使用无标记形式评估时，对血清中的抗牛血清白蛋白和兔免疫球蛋白表现出优异的选择性和灵敏度。值得注意的是，新材料在提供显著改善的韧性和弹性的同时，匹配了基于静态网络的传统水凝胶的分析性能，其压缩模量与人皮肤相当。这证明了滑环水凝胶在制造坚固的无标记生物传感平台方面的潜力。此外，该系统的灵活性允许纳入针对特定应用定制的各种识别元件。
