Goldstein H, Volkman D J, Ambrus J L, Fauci A S
J Immunol. 1985 Jul;135(1):339-43.
A human T4+/Leu-8+ T cell clone (YA2) was established by phytohemagglutinin activation and interleukin 2 (IL 2) propagation. Functional characterization of this clone demonstrated that it provided potent help towards Ig production by pokeweed mitogen-stimulated B cells in the presence of small numbers of autologous T cells or by Staphylococcus aureus Cowan I (SAC)-activated B cells in the presence of B cell growth factor (BCGF). YA2 provided no help to resting B cells and minimal help to either unactivated B cells cultured with BCGF or SAC-activated B cells. Supernatant generated from clone YA2 by IL 2 stimulation had significant B cell differentiation activity but no BCGF or IL 2 activity. Thus, YA2 is a T4+/Leu-8+ potent direct helper only to B cells that are activated and proliferating due to its selective secretion of a differentiation factor, and not an activation and growth factor. The availability of phenotypically defined cloned populations of T cells with restricted functional helper activity related to the secretion of selected B cell tropic factors should prove useful in the dissection of the role of individual T cell subsets in the regulation of the human B cell cycle.
通过植物血凝素激活和白细胞介素2（IL-2）增殖建立了一株人T4+/Leu-8+ T细胞克隆（YA2）。对该克隆的功能特性进行表征表明，在少量自体T细胞存在的情况下，它能为商陆有丝分裂原刺激的B细胞产生Ig提供有力帮助；或者在B细胞生长因子（BCGF）存在的情况下，为金黄色葡萄球菌Cowan I（SAC）激活的B细胞产生Ig提供有力帮助。YA2对静止B细胞没有帮助，对用BCGF培养的未激活B细胞或SAC激活的B细胞提供的帮助也很少。IL-2刺激克隆YA2产生的上清液具有显著的B细胞分化活性，但没有BCGF或IL-2活性。因此，YA2是一种T4+/Leu-8+细胞，它仅对因选择性分泌分化因子而被激活和增殖的B细胞具有强大的直接辅助作用，而不是激活和生长因子。具有与所选B细胞嗜性因子分泌相关的受限功能辅助活性的表型明确的T细胞克隆群体的可用性，应有助于剖析单个T细胞亚群在人类B细胞周期调节中的作用。
