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使用单克隆抗体WR16鉴定和分离OKT4 +抑制细胞。

Identification and isolation of OKT4+ suppressor cells with the monoclonal antibody WR16.

作者信息

Moore K, Nesbitt A M

出版信息

Immunology. 1986 Aug;58(4):659-64.

PMID:2942465
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC1453118/
Abstract

The monoclonal antibody WR16 was secreted by a hybridoma produced by fusing splenocytes from a BALB/c mouse immunized with human T-cell chronic lymphocytic leukaemia cells and the murine myeloma cell line NS-O. WR16 reacts specifically with human lymphocytes and binds to 48% of OKT4+ T lymphocytes and the majority of B lymphocytes. Human OKT4+ tonsil lymphocytes were subfractionated into WR16-/OKT4+ and WR16+/OKT4+ subpopulations by the panning technique. The capacity of these cells to help or suppress pokeweed mitogen-induced immunoglobulin (Ig) secretion by autologous B lymphocytes was monitored after 9 days of coculture. Cells of phenotype WR16-/OKT4+ enhanced Ig secretion in excess of that found with non-fractionated OKT4+ lymphocytes, whilst WR16+/OKT4+ lymphocytes suppressed Ig secretion when added to a mixture of B lymphocytes and non-fractionated OKT4+ cells. The WR16-/OKT4+ subpopulation was further fractionated by use of the MoAb Leu 8. Forty-two percent of WR16-/OKT4+ lymphocytes bound Leu 8, and cells of the phenotype WR16-/OKT4+/Leu 8+ were found to induce B-lymphocyte Ig secretion, whilst WR16-/OKT4+/Leu 8- lymphocytes were less active in this system. These data confirm the heterogeneity of the human T helper/inducer subset and indicate the existence of a population of OKT4+ lymphocytes that can suppress Ig secretion in the absence of OKT8+ lymphocytes.

摘要

单克隆抗体WR16由一种杂交瘤分泌，该杂交瘤是通过将用人类T细胞慢性淋巴细胞白血病细胞免疫的BALB/c小鼠的脾细胞与鼠骨髓瘤细胞系NS - O融合产生的。WR16与人淋巴细胞特异性反应，能与48%的OKT4 + T淋巴细胞以及大多数B淋巴细胞结合。通过淘选技术将人类OKT4 +扁桃体淋巴细胞亚分为WR16 - /OKT4 +和WR16 + /OKT4 +亚群。共培养9天后，监测这些细胞帮助或抑制美洲商陆有丝分裂原诱导的自体B淋巴细胞免疫球蛋白（Ig）分泌的能力。表型为WR16 - /OKT4 +的细胞增强了Ig分泌，超过了未分级的OKT4 +淋巴细胞；而WR16 + /OKT4 +淋巴细胞加入B淋巴细胞和未分级的OKT4 +细胞混合物中时则抑制Ig分泌。利用单克隆抗体Leu 8对WR16 - /OKT4 +亚群进一步分级。42%的WR16 - /OKT4 +淋巴细胞结合Leu 8，发现表型为WR16 - /OKT4 + /Leu 8 +的细胞可诱导B淋巴细胞Ig分泌，而WR16 - /OKT4 + /Leu 8 -淋巴细胞在该系统中的活性较低。这些数据证实了人类T辅助/诱导亚群的异质性，并表明存在一群OKT4 +淋巴细胞，在没有OKT8 +淋巴细胞的情况下能够抑制Ig分泌。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/aeb9/1453118/4cacac954355/immunology00181-0126-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/aeb9/1453118/4cacac954355/immunology00181-0126-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/aeb9/1453118/4cacac954355/immunology00181-0126-a.jpg

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本文引用的文献

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Heterogeneity of human T4+ inducer T cells defined by a monoclonal antibody that delineates two functional subpopulations.由一种单克隆抗体所界定的人类T4 +诱导性T细胞的异质性，该单克隆抗体划分出两个功能亚群。
基于L-C/T200抗原差异表达对T4（CD4）亚群进行细分。
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