DANYLO HALYTSKY LVIV NATIONAL MEDICAL UNIVERSITY, LVIV, UKRAINE; UNIVERSITY OF ZIELONA GÓRA, ZIELONA GÓRA, POLAND.
DANYLO HALYTSKY LVIV NATIONAL MEDICAL UNIVERSITY, LVIV, UKRAINE.
Wiad Lek. 2024;77(8):1603-1610. doi: 10.36740/WLek202408110.
Aim: The aim of the study was to determine the impact of cranioskeletal trauma (CST) on the development of endogenous intoxication syndrome in rats of different ages.
Materials and Methods: The experiments involved 147 white male Wistar rats of different age groups. The first experimental group included sexual immature rats aged 100-120 days. The second group includes sexually mature rats aged 6-8 months. The third group included old rats aged 19-23 months. In all experimental groups, CST was modelled under thiopentalonatrium anaesthesia. The control rats were only injected with thiopentalonatrium anaesthesia. The animals were withdrawn from the experiments under anaesthesia after 1, 3, 7, 14, 21 and 28 days by total bleeding from the heart. In blood serum, the content of fractions of molecules of middle mass was determined at a wavelength of 254 and 280 nm (MMM254, MMM280).
Results: As a result application of CST in rats of different age groups, an increase in the serum content of MMM254 and MMM280 was observed with a maximum after 14 days and a subsequent decrease by 28 days. At all times of the experiment, the indicators were statistically significantly higher compared to the control groups. The degree of growth of the MMM254 fraction after 1, 7 and 14 days was statistically significantly higher in sexual immature rats, and after 21 and 28 days - in old rats. In old rats after 21 and 28 days of the post-traumatic period, the content and degree of growth of the MMM280 fraction in the blood serum were also significantly higher.
Conclusions: Modelling of CST in rats of different age groups is accompanied by the development of endogenous intoxication syndrome, which is manifested by the accumulation of MMM254 and MMM280 fractions in the blood serum with a maximum after 14 days of the experiment. The content of the serum fraction of MMM254 in sexual immature rats in the dynamics of experimental CST exceeds other age groups after 1, 7 and 14 days, in old rats the content of the studied MMM fractions is significantly higher after 21-28 days.
本研究旨在确定颅面骨骼创伤（CST）对不同年龄大鼠内源性中毒综合征发展的影响。
实验涉及了 147 只不同年龄组别的雄性 Wistar 大鼠。第一实验组包括 100-120 天大的未成熟雄性大鼠。第二组包括 6-8 个月大的成熟雄性大鼠。第三组包括 19-23 个月大的老年大鼠。在所有实验组中，均在硫喷妥钠麻醉下建立 CST 模型。对照组大鼠仅接受硫喷妥钠麻醉注射。在麻醉下，所有动物在第 1、3、7、14、21 和 28 天通过心脏全血抽取退出实验。在血清中，在 254nm 和 280nm 波长下测定中分子物质的分数含量（MMM254、MMM280）。
结果：在不同年龄组大鼠中应用 CST 后，观察到血清中 MMM254 和 MMM280 的含量增加，在第 14 天达到最大值，随后在第 28 天下降。在整个实验期间，与对照组相比，这些指标均具有统计学显著差异。在 1、7 和 14 天时，未成熟大鼠的 MMM254 分数的增长程度具有统计学显著差异，而在 21 和 28 天时，老年大鼠则具有统计学显著差异。在创伤后 21 和 28 天的老年大鼠中，血清中 MMM280 分数的含量和增长程度也显著增加。
在不同年龄组大鼠中建立 CST 模型会导致内源性中毒综合征的发展，这表现为血清中 MMM254 和 MMM280 分数的积累，在实验的第 14 天达到最大值。在 CST 的实验动态中，未成熟雄性大鼠的血清 MMM254 分数含量在第 1、7 和 14 天超过其他年龄组，而在老年大鼠中，在第 21-28 天，研究中的 MMM 分数含量明显更高。
