I. Horbachevsky Ternopil National Medical University, Ternopil, Ukraine.
Folia Med (Plovdiv). 2023 Feb 28;65(1):149-154. doi: 10.3897/folmed.65.e71970.
Excessive lipoperoxidation is accompanied by accumulation of peroxidation products and depletion of antioxidant re-serves, which cause hyperenzymemia and the accumulation of toxic substances. The level of endotoxicosis is determined by the content of hydrophilic and hydrophobic products in the blood.
To define the pathogenetic role of endogenous intoxication in the dynamics of development of experimental periodontitis of bacterial-immune genesis.
The experiment was conducted on rats. The animals were divided into three groups: group 1 - control group/intact; group 2 - model periodontitis at 7 days; group 3 - model periodontitis at 30 days. The experimental bacterial-immune periodon-titis was induced by injection into the tissue of the periodontal complex of the microorganisms' mixture diluted with egg's albumin. For the study, we selected the blood serum in which the content of middle molecular weight molecules and erythrocyte intoxication index were determined. The results were statistically analyzed by means of non-parametric indices methods.
The development of generalized periodontitis is characterized by the occurrence of oxidative stress, which leads to violation of the metabolism in the mucous membrane of the oral cavity resulting in the accumulation of toxic products and the development of endogenous intoxication. The results show that the content of middle molecular weight molecules (aromatic amino and chain amino acids) determined on day 7 of the experiment was 1.11 times higher than that of the control group (p<0.01) and by 1.16 times (p<0.01), respectively. Comparing the levels of the above hydrophilic components of endogenous intoxication at 30 days of experimental peri-odontitis, we found a probable increase in these indicators compared with those at 7 days of the experiment. Studying the level of eryth-rocyte intoxication index, we found that at 7 days of experimental periodontitis this index was 1.28 times higher (p<0.01) than that of the intact group and continued to increase at 30 days.
The dynamics of experimental periodontitis of bacterial-immune genesis show that the highest rates of endogenous intoxication are found in the late stages of the dynamics of the inflammatory process in the periodontium, namely at 30 days of the experiment, which may indicate chronic inflammation.
过度的脂质过氧化会伴随着过氧化产物的积累和抗氧化储备的消耗，这会导致酶血症和有毒物质的积累。内毒素血症的水平取决于血液中亲水性和疏水性产物的含量。
确定内毒素中毒在细菌免疫起源的实验性牙周炎发展中的发病机制作用。
该实验在大鼠身上进行。将动物分为三组：第 1 组 - 对照组/完整；第 2 组 - 第 7 天的模型牙周炎；第 3 组 - 第 30 天的模型牙周炎。通过向牙周复合体组织中注射稀释的卵白蛋白的微生物混合物来诱导实验性细菌性免疫性牙周炎。为了研究，我们选择了血清，其中测定了中分子量分子和红细胞中毒指数的含量。使用非参数指标方法对结果进行了统计分析。
全身性牙周炎的发展特点是氧化应激的发生，这导致了口腔黏膜代谢的破坏，导致有毒产物的积累和内毒素中毒的发展。结果表明，在实验的第 7 天测定的中分子量分子（芳香族氨基酸和链状氨基酸）的含量比对照组高 1.11 倍（p<0.01），分别为 1.16 倍（p<0.01）。比较实验性牙周炎 30 天时上述亲水性内毒素成分的水平，我们发现与实验第 7 天时相比，这些指标可能有所增加。研究红细胞中毒指数的水平，我们发现实验性牙周炎的第 7 天，这个指数比完整组高 1.28 倍（p<0.01），并在第 30 天继续增加。
细菌免疫起源的实验性牙周炎的动态变化表明，内毒素中毒的最高率出现在牙周炎症过程的后期，即实验的第 30 天，这可能表明慢性炎症。
