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离体大鼠肝细胞对2-乙酰氨基芴的代谢活化。不同代谢产物与DNA修复及细菌诱变的关联。

Metabolic activation of 2-acetylaminofluorene by isolated rat liver cells. Involvement of different metabolites causing DNA-repair and bacterial mutagenesis.

作者信息

Brouns R M, Bos R P, von Doorn R, Henderson P T

出版信息

Arch Toxicol. 1980 May;45(1):53-9. doi: 10.1007/BF00303295.

DOI:10.1007/BF00303295
PMID:6994684
Abstract

Isolated rat liver cells are able to metabolize 2-acetylaminofluorene (2-AAF) to reactive species, capable of producing mutagenic effects in Salmonella typhimurium TA 1518 and evoking unscheduled DNA synthesis within the hepatocytes. Indications are presented, that these genotoxic effects are caused by different reactive metabolites. Mutagenesis could be blocked almost completely by paraoxon, an inhibitor of the de-acetylation reaction, whereas induction of DNA excision repair was prevented by antagonizing the sulfation reaction by means of salicylamide.

摘要

分离出的大鼠肝细胞能够将2-乙酰氨基芴（2-AAF）代谢为活性物质，这些活性物质能够在鼠伤寒沙门氏菌TA 1518中产生致突变作用，并在肝细胞内引发DNA的非预定合成。有迹象表明，这些遗传毒性效应是由不同的活性代谢物引起的。对脱乙酰化反应具有抑制作用的对氧磷几乎可以完全阻断诱变作用，而通过水杨酰胺拮抗硫酸化反应则可以防止DNA切除修复的诱导。

相似文献

1
Metabolic activation of 2-acetylaminofluorene by isolated rat liver cells. Involvement of different metabolites causing DNA-repair and bacterial mutagenesis.离体大鼠肝细胞对2-乙酰氨基芴的代谢活化。不同代谢产物与DNA修复及细菌诱变的关联。
Arch Toxicol. 1980 May;45(1):53-9. doi: 10.1007/BF00303295.
2
Metabolic activation of 2-aminofluorene by isolated rat liver cells through different pathways leading to hepatocellular DNA-repair and bacterial mutagenesis.分离的大鼠肝细胞通过不同途径对2-氨基芴进行代谢活化，导致肝细胞DNA修复和细菌诱变。
Toxicology. 1981;19(1):67-75. doi: 10.1016/0300-483x(81)90066-4.
3
Species differences in the cytotoxic and genotoxic effects of 2-acetylaminofluorene and its primary metabolites 2-aminofluorene and N-OH-2-acetylaminofluorene.2-乙酰氨基芴及其主要代谢产物2-氨基芴和N-羟基-2-乙酰氨基芴的细胞毒性和基因毒性作用的种属差异。
Carcinogenesis. 1985 Mar;6(3):421-5. doi: 10.1093/carcin/6.3.421.
4
Modulation of cytotoxic and genotoxic effects of 2-acetylaminofluorene in rat and hamster hepatocytes by 3-methylcholanthrene pre-treatment.3-甲基胆蒽预处理对2-乙酰氨基芴在大鼠和仓鼠肝细胞中细胞毒性和遗传毒性作用的调节
Carcinogenesis. 1986 Sep;7(9):1561-7. doi: 10.1093/carcin/7.9.1561.
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Modulation of genotoxic and cytotoxic effects of aromatic amines in monolayers of rat hepatocytes.大鼠肝细胞单层中芳香胺遗传毒性和细胞毒性作用的调节
Cell Biol Toxicol. 1984 Oct;1(1):95-110. doi: 10.1007/BF00125568.
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Analysis of rat lymphocyte activation of benzo[a]pyrene, 2-acetylaminofluorene, and several of their metabolites to mutagenic and DNA-damaging species in vitro.大鼠淋巴细胞对苯并[a]芘、2-乙酰氨基芴及其几种代谢产物在体外激活为诱变和DNA损伤物种的分析。
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Studies on the metabolic activation of benzidine by isolated rat hepatocytes.大鼠离体肝细胞对联苯胺的代谢活化研究。
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Mutagenicity and DNA-excision repair induced by isoniazid after metabolic activation by isolated human and rat hepatocytes.异烟肼经分离的人及大鼠肝细胞代谢活化后诱导的致突变性和DNA切除修复
Cancer Lett. 1986 Jan;30(1):103-11. doi: 10.1016/0304-3835(86)90139-4.
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Comparison of human and rat hepatocyte metabolism and mutagenic activation of 2-acetylaminofluorene.人及大鼠肝细胞对2-乙酰氨基芴的代谢及诱变激活作用的比较
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引用本文的文献

1
Retinol (vitamin A) as a modifier of 2-aminofluorene and 2-acetyl-aminofluorene mutagenesis in the Salmonella/microsome assay.视黄醇（维生素A）在沙门氏菌/微粒体试验中作为2-氨基芴和2-乙酰氨基芴诱变作用的调节剂。
Arch Toxicol. 1982 Jan;49(2):169-74. doi: 10.1007/BF00332364.
2
Sex differences in the biotransformation of 2-acetylaminofluorene in cultured rat hepatocytes.培养的大鼠肝细胞中2-乙酰氨基芴生物转化的性别差异。
Cell Biol Toxicol. 1986 Jun;2(2):271-81. doi: 10.1007/BF00122695.
3
Effects of vitamin A on the metabolism and mutagenicity of 2AAF in vitro and on the covalent binding to rat liver DNA/RNA in vivo.

本文引用的文献

1
N-hydroxy-2-acetylaminofluorene sulfotransferase: its probable role in carcinogenesis and in protein-(methion-S-yl) binding in rat liver.N-羟基-2-乙酰氨基芴磺基转移酶：其在大鼠肝脏致癌作用及蛋白质-（甲硫氨酸-S-基）结合中的可能作用
Cancer Res. 1970 Mar;30(3):577-95.
2
High-yield preparation of isolated rat liver parenchymal cells: a biochemical and fine structural study.大鼠肝脏实质细胞分离的高效制备：生化与精细结构研究
J Cell Biol. 1969 Dec;43(3):506-20. doi: 10.1083/jcb.43.3.506.
3
Preparation of rat liver cells. 3. Enzymatic requirements for tissue dispersion.
维生素A对2-乙酰氨基芴在体外的代谢和致突变性以及在体内与大鼠肝脏DNA/RNA共价结合的影响。
Arch Toxicol. 1985 Aug;57(3):178-83. doi: 10.1007/BF00290884.
大鼠肝细胞的制备。3. 组织分散的酶学要求。
Exp Cell Res. 1973 Dec;82(2):391-8. doi: 10.1016/0014-4827(73)90357-1.
4
Detection of carcinogens as mutagens in the Salmonella/microsome test: assay of 300 chemicals.在沙门氏菌/微粒体试验中作为诱变剂的致癌物检测：300种化学物质的测定
Proc Natl Acad Sci U S A. 1975 Dec;72(12):5135-9. doi: 10.1073/pnas.72.12.5135.
5
The use of liver epithelial cultures for the study of chemical carcinogenesis.利用肝上皮细胞培养物研究化学致癌作用。
Am J Pathol. 1976 Dec;85(3):739-54.
6
Methods for detecting carcinogens and mutagens with the Salmonella/mammalian-microsome mutagenicity test.用沙门氏菌/哺乳动物微粒体诱变性试验检测致癌物和诱变剂的方法。
Mutat Res. 1975 Dec;31(6):347-64. doi: 10.1016/0165-1161(75)90046-1.
7
Studies on the mutagenicity of N-hydroxy-2-acetylaminofluorene in the Ames-Salmonella mutagenesis test system.在埃姆斯-沙门氏菌诱变试验系统中对N-羟基-2-乙酰氨基芴致突变性的研究。
Biochem Pharmacol. 1978;27(20):2399-408. doi: 10.1016/0006-2952(78)90351-9.
8
Mutagenic activation of N-hydroxy-2-acetylaminofluorene in the Salmonella test system: the role of deacetylation by liver and kidney fractions from mouse and rat.N-羟基-2-乙酰氨基芴在沙门氏菌测试系统中的诱变激活：小鼠和大鼠肝脏及肾脏组分脱乙酰作用的影响
Mol Pharmacol. 1978 Jul;14(4):682-92.
9
Unscheduled DNA synthesis induced by procarcinogens in suspensions and primary cultures of hepatocytes on collagen membranes.原致癌物在胶原膜上肝细胞悬浮液和原代培养物中诱导的非预定DNA合成。
Cancer Res. 1978 Jul;38(7):1866-71.
10
Detection of chemical carcinogens by unscheduled DNA synthesis in rat liver primary cell cultures.通过大鼠肝脏原代细胞培养中的程序外DNA合成检测化学致癌物。
Cancer Res. 1977 Jun;37(6):1845-51.