采用健壮的米曲霉 BCC7051 进行油脂生产及其描述生长和油脂表型特征的数学模型。

Lipid production by robust Aspergillus oryzae BCC7051 and a mathematical model describing its growth and lipid phenotypic traits.

机构信息

Industrial Bioprocess Technology Research Team, Functional Ingredients and Food Innovation Research Group, National Center for Genetic Engineering and Biotechnology (BIOTEC), National Science and Technology Development Agency (NSTDA), Thailand Science Park, Khlong Nueng, Khlong Luang, Pathum Thani 12120, Thailand.

出版信息

J Appl Microbiol. 2024 Sep 2;135(9). doi: 10.1093/jambio/lxae229.

DOI:10.1093/jambio/lxae229

PMID:39231805

Abstract

AIMS

To identify the promising oleaginous Aspergillus oryzae strain and leverage its lipid and biomass production through a mathematical model.

METHODS AND RESULTS

Comparative profiling of the cell growth and total fatty acid (TFA) content among 13 strains of A. oryzae was performed to explore the discrimination in their lipid productions. The oleaginicity of A. oryzae was found to be strain dependent, where the fungal strain BCC7051 exhibited superior performance in producing lipid-rich biomass by submerged fermentation. The TFA contents of the strain BCC7051 were comparable when cultivated at a range of pH values (pH 3.5-6.5) and temperatures (24-42°C). The mathematical model was generated, well describing and predicting the fungal growth and lipid phenotypic traits at various temperatures and carbon substrates.

CONCLUSION

The A. oryzae strain BCC7051 was a robust cell factory, acquiring economically feasible options for producing valuable lipid-based products.

摘要

目的

通过数学模型，确定有前景的产油米曲霉菌株，并利用其生产油脂和生物质。

方法和结果

对 13 株米曲霉的细胞生长和总脂肪酸 (TFA) 含量进行比较分析，以探讨其产油能力的差异。结果表明，米曲霉的产油能力与菌株有关，真菌菌株 BCC7051 在深层发酵生产富含油脂的生物质方面表现出优异的性能。当在 pH 值（3.5-6.5）和温度（24-42°C）范围内培养时，菌株 BCC7051 的 TFA 含量相当。该模型生成后，可很好地描述和预测不同温度和碳源下真菌的生长和油脂表型特征。