College of Agronomy, Hunan Agricultural University, Changsha 410128, China; College of Bioscience and Biotechnology, Hunan Agricultural University, Changsha 410128, China.
College of Agronomy, Hunan Agricultural University, Changsha 410128, China.
Ecotoxicol Environ Saf. 2024 Oct 1;284:116974. doi: 10.1016/j.ecoenv.2024.116974. Epub 2024 Sep 3.
Biochar has been recognised as an efficacious amendment for the remediation of compound heavy metal contamination in soil. However, the molecular mechanism of biochar-mediated tolerance to compound heavy metal toxicity in cotton is unknown. The objective of this research was to investigate the positive impact of biochar (10 g·kg) on reducing damage caused by compound heavy metals (Cd, Pb, and As) in cotton (Gossypium hirsutum L.). The results revealed that biochar reduced Cd concentrations by 24.9 % (roots), and decreased Pb concentrations by 37.1 % (roots) and 59.53 % (stems). Biochar maintained ionic homoeostasis by regulating the expression of metal transporter proteins such as ABC, HIPP, NRAMP3, PCR, and ZIP, and genes related to the carbon skeleton and plasma membrane. Biochar also downregulated genes related to photosynthesis, thereby increasing photosynthesis. Biochar re-established redox homoeostasis in cotton by activating signal transduction, which regulated the activity of the enzymes POD, SOD, and CAT activity; and the expression of related genes. This research revealed the molecular mechanism by which biochar confers resistance to the harmful effects of compound heavy metal toxicity in cotton. The application of biochar as a soil amendment to neutralise the toxicity of compound heavy metals is recommended for cash crop production.
生物炭已被认为是修复土壤中复合重金属污染的有效改良剂。然而，生物炭介导棉花耐受复合重金属毒性的分子机制尚不清楚。本研究旨在探讨生物炭（10g·kg）对减轻棉花（Gossypium hirsutum L.）复合重金属（Cd、Pb 和 As）毒害的积极影响。结果表明，生物炭降低了根中 24.9%的 Cd 浓度，降低了根中 37.1%和茎中 59.53%的 Pb 浓度。生物炭通过调节 ABC、HIPP、NRAMP3、PCR 和 ZIP 等金属转运蛋白和与碳骨架和质膜相关的基因的表达，维持离子体内平衡。生物炭还下调了与光合作用相关的基因，从而提高了光合作用。生物炭通过激活信号转导，在棉花中重新建立了氧化还原平衡，调节了 POD、SOD 和 CAT 活性相关酶的活性；并调节了相关基因的表达。本研究揭示了生物炭赋予棉花抵抗复合重金属毒性有害影响的分子机制。建议将生物炭作为土壤改良剂应用于经济作物生产，以中和复合重金属的毒性。
