Chemical Engineering Department, Federal University of Ceara, 60440-900, Fortaleza, CE, Brazil.
Food Engineering Department, Federal University of Ceara, 60440-900, Fortaleza, CE, Brazil.
Food Res Int. 2024 Oct;194:114910. doi: 10.1016/j.foodres.2024.114910. Epub 2024 Aug 11.
This study aimed to optimize the ultrasound-assisted extraction (UAE) of anthocyanins from oven-dried and freeze-dried Vitis labrusca grape pomace, using acidified water as the solvent. The effects of power density (8.3-16.7 W/mL), pulse interval (0-2 s), and extraction time (1-5 min) on both total and specific anthocyanins were investigated. The findings suggested that acidified water can be a viable alternative to conventional solvents and that oven drying was an effective method for drying the pomace. Using response surface methodology, the study identified power density and extraction time as key factors influencing total anthocyanin content, with extracts reaching contents up to 2.56 mg/g. The analysis using LC-MS identified 14 anthocyanins, while NMR quantified 3 and malvidin diglucoside was generally the most abundant. However, higher power and longer extraction times were found to reduce its content while increasing malvidin monoglucoside content, suggesting ultrasound-induced anthocyanin hydrolysis. In conclusion, this study presents a sustainable method for extracting anthocyanins using acidified water, contributing to the valorization of Vitis labrusca grape pomace for industrial use.
本研究旨在优化超声辅助提取（UAE）酸水溶剂中干燥和冻干的葡萄渣中的花色苷，使用酸水作为溶剂。考察了功率密度（8.3-16.7 W/mL）、脉冲间隔（0-2 s）和提取时间（1-5 min）对总花色苷和特定花色苷的影响。结果表明，酸水可以替代传统溶剂，并且烘干是干燥渣的有效方法。利用响应面法，研究确定了功率密度和提取时间是影响总花色苷含量的关键因素，提取物中花色苷含量高达 2.56mg/g。LC-MS 分析鉴定出 14 种花色苷，而 NMR 定量分析出 3 种，其中矢车菊素二葡萄糖苷通常含量最丰富。然而，较高的功率和较长的提取时间会降低其含量，同时增加矢车菊素单葡萄糖苷的含量，表明超声诱导的花色苷水解。总之，本研究提出了一种使用酸水提取花色苷的可持续方法，为葡萄渣的工业利用提供了一种有价值的方法。
