Department of Food Science, University of Arkansas, 2650 North Young Avenue, Fayetteville, Arkansas 72704.
J Agric Food Chem. 2010 Mar 10;58(5):2862-8. doi: 10.1021/jf904087n.
Accelerated solvent extraction (ASE) was used to optimize and determine the effectiveness of an alternative, environmentally friendly extraction procedure using subcritical solvents to recover anthocyanins from freeze-dried, ground Sunbelt red grape pomace. Anthocyanins were extracted from pomace using the following ASE variables: pressure (6.8 MPa), one extraction cycle, and temperature (40, 60, 80, 100, 120, and 140 degrees C). Conventional solvent extraction with methanol/water/formic acid (60:37:3 v/v/v) was compared to four hydroethanolic solvents (10, 30, 50, and 70% ethanol in water, v/v). Anthocyanins in the extracts were identified and quantified by HPLC-MS and HPLC. There was an insignificant interaction between solvent and temperature (p = 0.0663). Solvents containing 70 and 50% ethanol in water extracted more total anthocyanins (463 and 455 mg/100 g of DW, respectively) than other solvents. The total amounts of anthocyanins extracted at 100 degrees C (450 mg/100 g of DW), 80 degrees C (436 mg/100 g of DW), and 120 degrees C (411 mg/100 g of DW) were higher than at the other temperatures. Solvents containing 70 and 50% ethanol in water extracted similar amounts of anthocyanins as conventional extraction solvent.
加速溶剂萃取（ASE）用于优化和确定替代的、环保的提取方法的有效性，该方法使用亚临界溶剂从冷冻干燥、研磨的 Sunbelt 红葡萄渣中回收花色苷。花色苷通过以下 ASE 变量从渣中提取：压力（6.8 MPa）、一个提取循环和温度（40、60、80、100、120 和 140°C）。与甲醇/水/甲酸（60:37:3v/v/v）的常规溶剂提取相比，比较了四种水醇溶剂（水相中的 10、30、50 和 70%乙醇，v/v）。提取物中的花色苷通过 HPLC-MS 和 HPLC 进行鉴定和定量。溶剂和温度之间的相互作用不显著（p=0.0663）。含 70%和 50%乙醇的水溶剂提取的总花色苷量（分别为 463 和 455mg/100g DW）高于其他溶剂。在 100°C（450mg/100g DW）、80°C（436mg/100g DW）和 120°C（411mg/100g DW）提取的总花色苷量高于其他温度。含 70%和 50%乙醇的水溶剂提取的花色苷量与常规提取溶剂相似。
