Morinan A, Garratt H M
J Pharmacol Methods. 1985 Jun;13(3):213-23. doi: 10.1016/0160-5402(85)90021-x.
A fluorimetric assay for monamine oxidase that uses kynuramine as the substrate is described. This method is more economic, rapid, reproducible, and sensitive than other similar procedures. Its validity has been confirmed by the study of the subcellular distribution, the effects of inhibitors, and the kinetics of enzyme activity. Previous reports of substrate inhibition by kynuramine may have to be reassessed in the light of evidence presented here.
本文描述了一种以犬尿胺为底物的单胺氧化酶荧光测定法。该方法比其他类似方法更经济、快速、可重复且灵敏。通过对亚细胞分布、抑制剂作用及酶活性动力学的研究，证实了其有效性。鉴于本文所提供的证据，先前关于犬尿胺底物抑制的报道可能需要重新评估。
