Department of Drug Discovery and Development, Harrison School of Pharmacy, Auburn University, Auburn, AL, USA.
Department of Pharmacology, Faculty of Medicine, University of Jeddah, Jeddah, KSA.
Vet Med Sci. 2020 Nov;6(4):766-774. doi: 10.1002/vms3.296. Epub 2020 Jul 6.
Recently, there is a significant increase in the commercial use of goat products. Nevertheless, there are very few reports on the characterization of redox biomarkers and mitochondrial function in the goat testis. Therefore, in this study we studied the markers of oxidative stress and mitochondrial functions in the goat testis during the process of ageing. Alterations in the markers of oxidative stress/redox biomarkers (contents of reactive oxygen species, nitrite, lipid peroxide, protein carbonyl, glutathione and activities of glutathione peroxidase, monoamine oxidase) and mitochondrial function (Complex-I and Complex-IV activities) were elucidated during the process of ageing. Augmented oxidative stress and decreased mitochondrial function were prominent during ageing in the goat testis. Ageing can lead to induction of oxidative stress and decreased production of ATP; however, the prooxidants generated must be effectively removed from the body by the innate antioxidant defence system to minimize the damage to the host tissue. Conversely, the antioxidants cannot completely scavenge the excessive amount of reactive oxygen species produced during ageing or pathological conditions leading to significant cell death and tissue damage. Thus, the use of effective and potent antioxidants in the feed could significantly reduce oxidative stress and improve mitochondrial function, resulting in enriched goat health.
最近，山羊产品的商业用途显著增加。然而，关于山羊睾丸中氧化还原生物标志物和线粒体功能的特征，却鲜有报道。因此，在本研究中，我们研究了山羊睾丸在衰老过程中氧化应激和线粒体功能的标志物。在衰老过程中，氧化应激/氧化还原生物标志物（活性氧、亚硝酸盐、脂质过氧化物、蛋白质羰基、谷胱甘肽含量和谷胱甘肽过氧化物酶、单胺氧化酶活性）和线粒体功能（复合物-I 和复合物-IV 活性）的标志物发生了改变。在山羊睾丸的衰老过程中，氧化应激增强，线粒体功能下降。衰老会导致氧化应激的诱导和 ATP 生成减少；但是，必须通过先天的抗氧化防御系统有效清除体内产生的促氧化剂，以将对宿主组织的损伤降至最低。相反，抗氧化剂不能完全清除衰老或病理条件下产生的过量活性氧，导致大量细胞死亡和组织损伤。因此，在饲料中使用有效和强效的抗氧化剂可以显著减轻氧化应激，改善线粒体功能，从而提高山羊的健康水平。
