French M C, Wishart G N
J Pharmacol Methods. 1985 Jun;13(3):241-8. doi: 10.1016/0160-5402(85)90024-5.
An improved technique for perfused lung preparations adapted for absorption studies with organic aerosols is described. The model developed by Niemeier and Bingham (1972) was modified to place greater emphasis on the respiratory functions of the lung as an index of viability. Perfusion of this preparation for 2 hr showed that tidal volume, pulmonary blood flow, and visual appearance were the most sensitive indicators of physiological change. The importance of relevant criteria for assessment of viability is discussed. In addition, a simple technique for on-line computerized measurement of tidal volume and respiratory frequency is described.
本文描述了一种经过改进的用于灌注肺制备的技术，该技术适用于有机气溶胶吸收研究。对Niemeier和Bingham（1972年）开发的模型进行了修改，以更加强调肺的呼吸功能作为生存能力的指标。对该制剂进行2小时灌注显示，潮气量、肺血流量和外观是生理变化最敏感的指标。讨论了评估生存能力的相关标准的重要性。此外，还描述了一种用于在线计算机测量潮气量和呼吸频率的简单技术。
