USDA-ARS, Molecular Plant Pathology Laboratory, Beltsville, MD, USA.
J Virol Methods. 2024 Dec;330:115021. doi: 10.1016/j.jviromet.2024.115021. Epub 2024 Sep 2.
This study describes production of polyclonal antibodies against recently reported novel potyvirid infecting alfalfa (Medicago sativa L.). The virus was first found in alfalfa seed material and later identified in plant samples collected from commercial alfalfa fields in Arizona, USA. It was classified as a novel species related to the members of the genus Ipomovirus and potentially representing a new genus in the family Potyviridae (Nemchinov et al., 2023b). Polyclonal antibodies were produced against the predicted viral coat protein expressed in bacterial cells and used in different types of immunoassays for specific detection of this emerging virus. They could be helpful in plant virus certification programs, screening of alfalfa germplasm, research on pathogenicity, biology, and geographic distribution of this emerging virus.
本研究描述了针对最近报道的新型马铃薯 Y 病毒属侵染紫花苜蓿（Medicago sativa L.）的多克隆抗体的制备。该病毒最初在紫花苜蓿种子材料中发现，后来在美国亚利桑那州的商业紫花苜蓿田中采集的植物样本中被鉴定出来。它被归类为与成员属马铃薯 Y 病毒属相关的新型种，可能代表了马铃薯病毒科中的一个新属（Nemchinov 等人，2023b）。针对在细菌细胞中表达的预测病毒外壳蛋白制备了多克隆抗体，并在不同类型的免疫分析中用于该新兴病毒的特异性检测。它们可能有助于植物病毒认证计划、紫花苜蓿种质资源筛选、该新兴病毒的致病性、生物学和地理分布研究。
